You want to digest 1 µg of plasmid DNA in a final volume of 50 μL. Your solutioncontaining plasmid has a concentration of 25 ng/μL. How many μL of your plasmidsolution do you need to add to your reaction tube to digest your desired mass ofplasmid?

A plasmid is an extrachromosomal DNA molecule within a cell that is distinct from chromosomal DNA…

Answered: You want to digest 1 µg of plasmid DNA…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

We use a NanoDrop spectrophotometer to analyze plasmid DNA obtained from miniprep. Whatinformation about the plasmid does the 260nm reading alone provide and why?
The gene atg-12 codes for a protein associated with abnormal rates of cell destruction and recycling of cell contents. Scientists studying bacterial plasmids devised an experiment using recombinant DNA techniques to remove a section of DNA (gene atg-12) of a bacterial plasmid (pOKE103) and create a new plasmid (pOKE104) that did not contain the gene atg-12. The new plasmid was then incorporated within the DNA of the fungus Neurospora crassa in cellular studies. Which statement explains the expected heredity of fungi that incorporate pOKE104? A - Fungi that incorporate pOKE104 will produce the protein from gene atg-12 and have increased rates of malignant tumors. B - Fungi that incorporate pOKE104 will not produce the protein from gene atg-12 but will have a normal appearance. C - The prokaryotic DNA will remain separate within the fungal cells, produce the protein from gene atg-12, and have increased rates of malignant tumors. D - The prokaryotic DNA will remain separate within the…
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Consider the image below. This image shows plasmid DNA isolated through exactly the same method that you used. The lanes that were untreated (TE) contain plasmid DNA, but also large amounts of smaller nucleic acids that are predicted to be RNA. What is the experimental evidence from the gel itself that these smaller molecules are RNA? (Hint: look at the treatments performed in each lane)
I currently have 6 stock plasmids that all have 2ug of material at 50ng/ul with a volume of 200ul each. How do I make a dilution from the stock with a volume of 1mL?
When you are loading the DNA ladder into your gel the volume needed is 5uL. The appropriate micropipette for this volume is a: 1)p2 2)p200 3)p1000 4)p20
After plasmid isolation, you take 0.5 uL of the sample and mix it with 1 uL of TE buffer. The absorbance of this sample at 260 nm is 0.8 and the average extinction coefficient of double-stranded DNA at 260 nm is 0.020 (μg/ml)−1 cm−1. What is the concentration of the undiluted sample? 120 μg/mL 80 μg/mL 40 μg/mL 12 μg/mL
Your plasmid miniprep has a concentration of 25 ng/µL and you add 30 µL to a digest tube. Your final reaction volume will be 50 µL. What is your final plasmid concentration in ng/µL?
Based on the image below, answer the following questions: Restriction enzymes PstI and ScaI were employed and the gel image after restriction digest is shown below. The name of the plasmid is Blank 1. The gel lane on the Blank 2 was digested using PstI enzyme. (left or right) The gel lane on the Blank 3 was digested using PstI and ScaI enzymes. (left or right)

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You want to digest 1 µg of plasmid DNA in a final volume of 50 µL. Your solution containing plasmid has a concentration of 25 ng/µL. How many µL of your plasmid solution do you need to add to your reaction tube to digest your desired mass of plasmid?