You want to digest 1 µg of plasmid DNA in a final volume of 50 μL. Your solutioncontaining plasmid has a concentration of 25 ng/μL. How many μL of your plasmidsolution do you need to add to your reaction tube to digest your desired mass ofplasmid?

A plasmid is an extrachromosomal DNA molecule within a cell that is distinct from chromosomal DNA…

Answered: You want to digest 1 µg of plasmid DNA…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

Give Detailed Solution with explanation (no need Handwritten)
I got these wrong can you please help? don’t give long explanations just help me finding right answers
What is a plasmid?
Would you expect transfer of chromosomal DNA by conjugation to be more efficient if cells were plated together on solid medium (agar) or mixed together in a liquid in a shaking flask? Explain.
What is a plasmid and what is its function?
Give a clear handwritten answer...?
help me with this multiple choice question please, this is molecular and cell biology Visualization of an electrophoresed agarose gel (D) Plasmid DNA cannot be visualized on an agarose gel (A) Plasmid DNA may be visualized as multiple supercoiled forms (B) Visualized plasmid DNA is always linear Ⓒ(C) Visualized plasmid DNA is always circular
Plate LB-Plasmid LB + Plasmid LB/amp - Plasmid Yes Yes No + LB LB/amp + Plasmid Yes LB Prediction Reeson Plate only contains Yes food/bacteria Plate only contains Yes food/bacteria Bacteria will be killed by ampicillin Bacteria has ampicillin resistance LB Click on pic to zoom Click on pic to zoom -LB Observed Result No Yes chlamp x Click on pic to zoom + LB/AMP Blamp Click on pic to zoom - LB/AMP
Plasmid alone should be ....... after adding the enzyme dna ligase. become circular single stranded degraded remain linear
Plate LB-Plasmid LB + Plasmid LB/amp - Plasmid Yes Yes No + LB LB/amp + Plasmid Yes LB Prediction Reeson Plate only contains Yes food/bacteria Plate only contains Yes food/bacteria Bacteria will be killed by ampicillin Bacteria has ampicillin resistance LB Click on pic to zoom Click on pic to zoom -LB Observed Result No Yes chlamp x Click on pic to zoom + LB/AMP Blamp Click on pic to zoom - LB/AMP
List three differences between a plasmid and a chromosome
2. pBR3322, an artificially made plasmid, is shown below. ECORI Hindill BamHI Pstl Amp ORI ↓↓ Tetr pBR322 (4.36 kb) ↑ Pvull Xmalll Describe 3 features that this plasmid has and indicate each one on the diagram. i) ii) 111]

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You want to digest 1 µg of plasmid DNA in a final volume of 50 µL. Your solution containing plasmid has a concentration of 25 ng/µL. How many µL of your plasmid solution do you need to add to your reaction tube to digest your desired mass of plasmid?