You want to digest 1 µg of plasmid DNA in a final volume of 50 μL. Your solutioncontaining plasmid has a concentration of 25 ng/μL. How many μL of your plasmidsolution do you need to add to your reaction tube to digest your desired mass ofplasmid?

A plasmid is an extrachromosomal DNA molecule within a cell that is distinct from chromosomal DNA…

Answered: You want to digest 1 µg of plasmid DNA…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The gene atg-12 codes for a protein associated with abnormal rates of cell destruction and recycling of cell contents. Scientists studying bacterial plasmids devised an experiment using recombinant DNA techniques to remove a section of DNA (gene atg-12) of a bacterial plasmid (pOKE103) and create a new plasmid (pOKE104) that did not contain the gene atg-12. The new plasmid was then incorporated within the DNA of the fungus Neurospora crassa in cellular studies. Which statement explains the expected heredity of fungi that incorporate pOKE104? A - Fungi that incorporate pOKE104 will produce the protein from gene atg-12 and have increased rates of malignant tumors. B - Fungi that incorporate pOKE104 will not produce the protein from gene atg-12 but will have a normal appearance. C - The prokaryotic DNA will remain separate within the fungal cells, produce the protein from gene atg-12, and have increased rates of malignant tumors. D - The prokaryotic DNA will remain separate within the…
List three important characteristics of plasmids and their function
What type of matrix was used to separate DNA in gel electrophoresis? Agarose 1X TBE Glycerol Polyacrylamide Ο 1X TAE
Consider the image below. This image shows plasmid DNA isolated through exactly the same method that you used. The lanes that were untreated (TE) contain plasmid DNA, but also large amounts of smaller nucleic acids that are predicted to be RNA. What is the experimental evidence from the gel itself that these smaller molecules are RNA? (Hint: look at the treatments performed in each lane)
When you are loading the DNA ladder into your gel the volume needed is 5uL. The appropriate micropipette for this volume is a: 1)p2 2)p200 3)p1000 4)p20
After plasmid isolation, you take 0.5 uL of the sample and mix it with 1 uL of TE buffer. The absorbance of this sample at 260 nm is 0.8 and the average extinction coefficient of double-stranded DNA at 260 nm is 0.020 (μg/ml)−1 cm−1. What is the concentration of the undiluted sample? 120 μg/mL 80 μg/mL 40 μg/mL 12 μg/mL
Your plasmid miniprep has a concentration of 25 ng/µL and you add 30 µL to a digest tube. Your final reaction volume will be 50 µL. What is your final plasmid concentration in ng/µL?
Why are plasmids dangerous?
Calculate the purity of the plasmid DNA. Do you think the plasmid is pure? Why?
What is a plasmid?
You have two DNA samples. Only one was expected to have plasmid DNA that was isolated from it. Both DNA samples were exposed to two different treatment conditions: untreated, or treated with DNase I. How should the DNase I treatment change how DNA appears on the gel?
Besides their role in genetic engineering, what else do plasmids can plasmids be used for? How are restriction enzymes used in genetic engineering?

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You want to digest 1 µg of plasmid DNA in a final volume of 50 µL. Your solution containing plasmid has a concentration of 25 ng/µL. How many µL of your plasmid solution do you need to add to your reaction tube to digest your desired mass of plasmid?