A plasmid comprised of B-DNA changed its linking number from 420 to 457, resulting in a superhelixdensity of 0.031. What is the length of the plasmid in base pairs?15050112003500125328765

Introduction: DNA in the cell must be arranged in order to allow the packing of large DNA molecules…

Answered: A plasmid comprised of B-DNA changed…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

What is a plasmid?
What is the size for cut lane #5 to measure the Sac1? The T-vector size is 3kb, what is the size for the insert?
An 9 kb circular plasmid is cut with the EcoRI restriction enzyme, and the reaction products are run on a DNA gel and stained with ethidium bromide. Bands of 1, 3 and 5 kb are seen. How many EcoRI sites are in the plasmid? Choose the one answer that is most correct. a) At least 2 b) At least 3 c) At least 1 d) None e) At least 4 f) At least 5
Give a clear handwritten answer...?
You are interested in cloning a gene that codes for an enzyme that produces a blue pigment. You have chosen to use the plasmid pUC19 for cloning (shown below). Drdl 91 Acll 2297 Xmnl 2294 Bcgl 2215 Scal 2177 Pvul 2066 Avall 2059 BsrDI 1935 Acll 1924 Fspl 1919 Avall 1837 NmeAIII 1822 Bgll 1813- Bpml 1784- BsrFI 1779 Bsal 17661 BsrDI 1753 Bmrl 1744 Aatll - Zral 2617 BriVI 2542 Sspl 2501- BsMBI 51 BsmBI 2683 Eco01091 2674 Ahdl 1694 PUC19 2,686 bp BceAl 1292 ori BstAPI 179 Ndel 183 Kasl - Narl - Sfol 235 Bell 245 Fspl 256 laczo AlwNI 1217 Pvul 276 Pvull 306 Bmrl 364 BseYI 1110 BceAl 387 MCS BeiVI 1015 Til 781 AflIlI-Pcil 806 Drdl 908 Apol-EcoRI 396 Banll - Sacl - Eco53KI 402 Acc651 - Kpnl 408 Aval-BsoBI- Smal- TspMI-Xmal 412 Bamil 417 Pvull 628 Tfil 641 BsaXI 659 BspQI-Sapl 683 Xbal 423 Accl - Hincll - Sall 429 BspMI-BfuAl 433 Sbfl 434 Pstl 435 Sphl 441 HindIII 447 The plasmid codes for three elements, lacZ, ApR and ori. What are these three things? Which restriction enzyme cut sites would…
SDS polyacrylamide electrophoresis was used to determine of the sizes of the wild type and Mutant 1 and 2 HOAP proteins expressed from these plasmids in bacteria. Using the Protein Standards as a guide, estimate the sizes of the HOAP protein expressed from each plasmid. 250 100 75 50 25 10

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A plasmid comprised of B-DNA changed its linking number from 420 to 457, resulting in a superhelix density of 0.031. What is the length of the plasmid in base pairs? 15050 11200 3500 12532 8765