A plasmid comprised of B-DNA changed its linking number from 420 to 457, resulting in a superhelixdensity of 0.031. What is the length of the plasmid in base pairs?15050112003500125328765

Introduction: DNA in the cell must be arranged in order to allow the packing of large DNA molecules…

Answered: A plasmid comprised of B-DNA changed…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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FIGURE 5 shows the base sequence of the DNA before and after mutation. Which of the following are CORRECT about the types of gene mutation? * Normal DNA: CGA TTA CCA TGC ATA CGA TTA ACC ATG CAT A CGA TTA CAC TGC ATA I III CGA TTA CCA GGC ATA II Mutant DNA Types of gene mutation I Base Deletion II Base Substitution II Base Inversion FIGURE 5 O I and Il only O I and III only Il and III only O I, Il and III
Clone number in this case is number 196 as shown in the images. What is the exact length of the segment of human DNA that has been inserted into the plasmid? *report the entire length of the insert, not just the sequences matching the ends and labels of wells isn't needed for answer*
Give a clear handwritten answer...?
Competent E. coli cells were transformed with the pGLO plasmid. These transformed cells were then allowed to grow on two different plates: 1) a plate containing LB/AMP and 2) another plate containing LB/AMP/ARA. In which plate would you observe both phenotypic and genotypic changes? Briefly justify your answer. Edit View Insert Format Tools Table 12pt v Paragraph v BIUA e T?v 田 D2
You are interested in cloning a gene that codes for an enzyme that produces a blue pigment. You have chosen to use the plasmid pUC19 for cloning (shown below). Drdl 91 Acll 2297 Xmnl 2294 Bcgl 2215 Scal 2177 Pvul 2066 Avall 2059 BsrDI 1935 Acll 1924 Fspl 1919 Avall 1837 NmeAIII 1822 Bgll 1813- Bpml 1784- BsrFI 1779 Bsal 17661 BsrDI 1753 Bmrl 1744 Aatll - Zral 2617 BriVI 2542 Sspl 2501- BsMBI 51 BsmBI 2683 Eco01091 2674 Ahdl 1694 PUC19 2,686 bp BceAl 1292 ori BstAPI 179 Ndel 183 Kasl - Narl - Sfol 235 Bell 245 Fspl 256 laczo AlwNI 1217 Pvul 276 Pvull 306 Bmrl 364 BseYI 1110 BceAl 387 MCS BeiVI 1015 Til 781 AflIlI-Pcil 806 Drdl 908 Apol-EcoRI 396 Banll - Sacl - Eco53KI 402 Acc651 - Kpnl 408 Aval-BsoBI- Smal- TspMI-Xmal 412 Bamil 417 Pvull 628 Tfil 641 BsaXI 659 BspQI-Sapl 683 Xbal 423 Accl - Hincll - Sall 429 BspMI-BfuAl 433 Sbfl 434 Pstl 435 Sphl 441 HindIII 447 The plasmid codes for three elements, lacZ, ApR and ori. What are these three things? Which restriction enzyme cut sites would…
Below is the 5’–3’ strand of a double-stranded DNA molecule with the following nucleotide sequences (all belong to an exon):5’ C C T A T G C A G T G G C C A T A T T C C A A A G C A T A G C 3’1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 311. If the above DNA strand is the coding (sense) strand and the DNA molecule is expressed to produce a protein product, however prior to expression, mutation took place where,a. the 15th base was replaced by Guanine. Is the amino acid sequence of the synthesized polypeptide chain altered, as compared when there was no mutation? Specify type of mutation in relation to protein function. Is the base substitution a transition or transversion mutation?b. the 18th base was replaced by Guanine. Is there an effect in the structure and function of the synthesized protein, as compared when there was no mutation? Specify type of mutation in relation to protein function. Is the base substitution a transition or transversion…
SDS polyacrylamide electrophoresis was used to determine of the sizes of the wild type and Mutant 1 and 2 HOAP proteins expressed from these plasmids in bacteria. Using the Protein Standards as a guide, estimate the sizes of the HOAP protein expressed from each plasmid. 250 100 75 50 25 10
Will you please help me in understanding these? How many fragments will be formed after digestion in sample C, if the plasmid is circular and why? What are the critical steps in transformation? How was RAD52 expression observed? Provide current applications of DNA Technology
In the lanes with the vector plasmid (lanes 7 and 8) label bands on the “sample” get that correspond to the following DNA forms: supercooled circles, relaxed circles, linear plasmid, and concatemers.
Dr. Wakefield would like to isolate recombinant plasmids from her bacterial culture using the alkaline lysis method. She is planning to use the chemicals as listed below: Solution I: 50 mM glucose, 25 mM Tris-Cl (pH 8.0), 10 mM EDTA (pH 8.0) Solution II: ??? Solution III: 5 M potassium acetate, glacial acetic acid, de-ion water Ethanol 70% (v/v) Isopropanol TE-RNAase pH 8.0 (i) (ii) (iii) Based on the chemical list above, state the content(s) of Solution II. Explain the functions of Solution II described in Q3 a) (i) in plasmid isolation. What is the role of alcohol precipitation conducted after the plasmids are obtained at the end of the procedure? Discuss the roles of ethanol and salt in alcohol precipitation step.
One feature that virtually all plasmid vectors have incommon is the polylinker (also called a multiple cloningsite). Explain what a polylinker is and why it is such animportant feature
Use the plasmid map for pBashi below to answer the following questions Xhol Hind!!! 15 kb 11 kb 10 kb 8 kb 7 kb 5 kb a. How large is pBashi? b. On the gel below, draw the bands corresponding to the expected pBashi cleavage products after restriction digest with the indicated restriction enzymes (Lane 2 - Xhol only, Lane 3 - HindIII only, Lane 4 - Xhol+ HindIII) 4 kb 3 kb 3 kb 2 kb 1 kb 4 kb Hindill 1 kb 7 kb Xhol Xhol Xhol Xhol Hind!!! Ladder Xhol Hindill Hind!!! Pstl Pstl c. Lane 5 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ Pstl. Lane 6 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ HindIII + Pstl. Indicate your final answers to the questions below clearly on the pBashi plasmid above; you will NOT receive any credit if you only list sizes of fragments below. If you need to, draft your answer somewhere else before writing your final answer above.…

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A plasmid comprised of B-DNA changed its linking number from 420 to 457, resulting in a superhelix density of 0.031. What is the length of the plasmid in base pairs? 15050 11200 3500 12532 8765