Eco01091 2074 Pfol 46 BstAPI 170 Aatil 2017 Sspl 2501 Ndel 183 Ehel 235 Pdml 2204, Bogi 2215 Soal 2172 MCS lacz 2480 230 PUC18/19 2686 bp Sapi 083 AI, Pacl s00 Gsul 1784 Ctri0l 1779 Eco311 1766 Eam11051 1094 1400 rep (pMB1) BoeYl 1110 \Cail 1217 1. You have the plasmid PUC18/19, which is a circular plasmid that consists of 2686 bp. What would the number of and length of the fragments be if you cut the plasmid with the following restriction enzymes or combination of enzymes? Give a schematic representation of the digestions. a. Pscl & Gsul b. Scal. Pdml & BsaXI c. Scal, Sspl & Ehel bla (Ap
Eco01091 2074 Pfol 46 BstAPI 170 Aatil 2017 Sspl 2501 Ndel 183 Ehel 235 Pdml 2204, Bogi 2215 Soal 2172 MCS lacz 2480 230 PUC18/19 2686 bp Sapi 083 AI, Pacl s00 Gsul 1784 Ctri0l 1779 Eco311 1766 Eam11051 1094 1400 rep (pMB1) BoeYl 1110 \Cail 1217 1. You have the plasmid PUC18/19, which is a circular plasmid that consists of 2686 bp. What would the number of and length of the fragments be if you cut the plasmid with the following restriction enzymes or combination of enzymes? Give a schematic representation of the digestions. a. Pscl & Gsul b. Scal. Pdml & BsaXI c. Scal, Sspl & Ehel bla (Ap
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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