Eco01091 2074 Pfol 46 BstAPI 170 Aatil 2017 Sspl 2501 Ndel 183 Ehel 235 Pdml 2204, Bogi 2215 Soal 2172 MCS lacz 2480 230 PUC18/19 2686 bp Sapi 083 AI, Pacl s00 Gsul 1784 Ctri0l 1779 Eco311 1766 Eam11051 1094 1400 rep (pMB1) BoeYl 1110 \Cail 1217 1. You have the plasmid PUC18/19, which is a circular plasmid that consists of 2686 bp. What would the number of and length of the fragments be if you cut the plasmid with the following restriction enzymes or combination of enzymes? Give a schematic representation of the digestions. a. Pscl & Gsul b. Scal. Pdml & BsaXI c. Scal, Sspl & Ehel bla (Ap

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Section B: Circular DNA
Plasmid map
Eco01091 2674 Pfol 46 BstAPI 179
Aatil 2617
Sspl 2501
Ndel 183
Ehel 235
Pdml 2204
MCS
lacz
146
Bogl 2215
Scal 2177
2486
PUC18/19
2686 bp
Bsax 659
Sapl 683
852
LATII, Pscl 806
Gsul 1784
1626
Cfri0l 1779
Eco311 1766
Eam11051 1094.
1400
rep (PMB1)
ACail 1217
\BseYl 1110
1. You have the plasmid PUC18/19, which is a circular plasmid that consists of 2686 bp.
What would the number of and length of the fragments be if you cut the plasmid with
the following restriction enzymes or combination of enzymes? Give a schematic
representation of the digestions.
a. Pscl & Gsul
b. Scal. Pdml & BsaXI
c. Scal. Sspl & Ehel
bla (Ap
Transcribed Image Text:Section B: Circular DNA Plasmid map Eco01091 2674 Pfol 46 BstAPI 179 Aatil 2617 Sspl 2501 Ndel 183 Ehel 235 Pdml 2204 MCS lacz 146 Bogl 2215 Scal 2177 2486 PUC18/19 2686 bp Bsax 659 Sapl 683 852 LATII, Pscl 806 Gsul 1784 1626 Cfri0l 1779 Eco311 1766 Eam11051 1094. 1400 rep (PMB1) ACail 1217 \BseYl 1110 1. You have the plasmid PUC18/19, which is a circular plasmid that consists of 2686 bp. What would the number of and length of the fragments be if you cut the plasmid with the following restriction enzymes or combination of enzymes? Give a schematic representation of the digestions. a. Pscl & Gsul b. Scal. Pdml & BsaXI c. Scal. Sspl & Ehel bla (Ap
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