Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy.IF the EcoRI/BamHI double digest produces 3 fragments with only two sizes, what are their sizes?2.5 kb, 250 b and 250 b 3.0 kb 2.0 kb 500 b and 500 b 3.0 kb 1.0kb and 1.0 kb ### Gel Electrophoresis Analysis of DNA FragmentsIn this section, we will analyze the results of a gel electrophoresis experiment using various restriction enzymes to cut DNA. The image displays a gel electrophoresis result with several lanes, each representing a different experimental condition.#### Lane Descriptions and Observations:- **L**: This lane contains a DNA ladder, which serves as a molecular weight marker. The ladder shows bands of known sizes, allowing estimation of DNA fragment sizes in other lanes. Marked reference bands include: - 3000 base pairs (bp) - 2000 bp - 1000 bp - 800 bp - 700 bp - 500 bp- **Uncut**: This lane contains the uncut DNA sample, which runs as a single band around the 3000 bp mark, indicating the size of the intact DNA fragment.- **EcoRI**: This lane shows the DNA sample digested with the EcoRI enzyme. The presence of bands closer to the 2000 bp and 1000 bp marks suggests that EcoRI cuts the DNA into two fragments of these approximate sizes.- **BamHI**: The DNA sample in this lane is digested with BamHI. The resulting fragments appear to be around 2000 bp and 1000 bp, similar to the EcoRI digestion pattern.- **NcoI**: DNA digested with NcoI shows distinct fragments. The prominent bands suggest NcoI cuts the DNA into unique-sized fragments. - **EcoRI/BamHI**: Double digestion with EcoRI and BamHI enzymes results in multiple bands, indicating additional cuts compared to single enzyme digestion, producing smaller fragments.- **BamHI/NcoI**: This lane represents DNA digested with both BamHI and NcoI, with several distinct fragments suggesting a more complex pattern of cleavage.- **EcoRI/NcoI**: Dual digestion with EcoRI and NcoI shows several bands, different from the patterns observed with individual enzymes, indicating more sites of cleavage.- **B/N/E**: Represents triple digestion of DNA with BamHI, NcoI, and EcoRI. This lane shows numerous small fragments, suggesting the most extensive digestion among all lanes and thus multiple cleavage sites.#### Analysis:The gel shows the migration of DNA fragments from all samples. The more enzyme sites present on the DNA, the more

Name: Use the gel to answer the following questions. You will be constructin
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Description: Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy.IF the EcoRI/BamHI double digest produces 3 fragments with only two sizes, what are their sizes?2.5 kb, 250 b and 250 b 3.0 kb 2.0 kb 500 b and 500