Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Choose the one answer that fits best. The pGLO plasmid you used contains an araC gene, a GFP gene and a bla gene. If you performed the exact same experiment with a plasmid that contained a GFP gene and a bla gene, but was missing the araC gene, what would you expect to see on your four plates?
| a. |
The positive control would not have any bacterial lawn
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| b. |
The negative control would have as many colonies as the experimental plate |
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| c. |
The LB/Amp/Ara plate would have some colonies but they would not be fluorescent under UV light
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| d. |
The experimental plate would not have any colonies |
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| e. |
The LB/Amp plate containing E. coli (+)pGLO would be fluorescent |
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