You are cloning a gene called ice, which will help strawberry plantssurvive in cold weather, into a test group of strawberry plants. Selectthe events you would use to genetically engineer these plants.Insert the ice gene into the T-DNA region of a Ti plasmid;transform Agrobacterium tumefaciens with this plasmid; Inoculate aculture of strawberry plant cells with Agrobacterium tumefaciens; Selectplant cells that have taken up the ice gene.Insert the ice gene into the T-DNA region of the Agrobacteriumtumefaciens chromosome; Inoculate a culture of strawberry plant cellswith Agrobacterium tumefaciens; Select plant cells that have taken upthe ice gene.O Insert the ice gene into a Ti transposon; Introduce the transposoninto Agrobacterium tumefaciens Ti plasmid: Inoculate a culture ofstrawberry plant cells with Agrobacterium tumefaciens: Select plant cellsthat have taken up the ice gene.Insert the ice gene into a Ti bacteriophage; transduce Agrobacteriumtumefaciens with this bacteriophage; Inoculate a culture of strawberryplant cells with Agrobacterium tumefaciens lysogens; Select plant cellsthat have taken up the ice gene.◄ PreviousNext ▸

Other incorrect options:B. This technique involves introducing the ice gene directly into the T-DNA…

Answered: You are cloning a gene called ice,…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Arrange the steps that would be used in a laboratory to engineer a bacterium that could express the human gene coding for factor VIII. Not all steps will be placed. Isolate the human gene that produces factor VIII. Isolate the mRNA of the factor VIII gene. Generate cDNA of the factor VIII gene using reverse transcriptase. Incorrect Insert the factor VIII cDNA into a bacterial vector near a promoter site. Transform the vector into an E. coli bacterium. Human DNA is introduced into the bacterial cell using direct injection by a small needle. E. coli expresses factor VIII. Answer Bank The factor VIII protein is isolated from human tissues.
For each of the features present on the plasmid (vector) indicate whether it is needed for use during clinging and replication in bacteria, GFP expression in eukaryotic cells, or not applicable: antibiotic resistance (BAC, GFP, or NA) multiple cloning sites (BAC, GFP, or NA) poly-A signal (BAC, GFP, or NA) promotor (BAC, GFP, or NA) GFP gene (BAC, GFP, or NA) origin (BAC, GFP, or NA) Pribnow box (BAC, GFP, or NA) TATA box (BAC, GFP, or NA)
You first need to create a plasmid. What are the minimal components necessary to develop this plasmid? In addition to these components, please draw the plasmid. In this illustration, I am looking for the components, the direction of transcription (i.e., the direction the genes will be transcribed), and what should be transcribed last? Also, where would you specifically insert the P. falciparum gene in this plasmid, and why are you checking reading frames in your gene? Finally, please name your plasmid using the correct nomenclature too. You are excited you have this new plasmid; you want to transfect it into P. marinus and provide it as an oral vaccine to laboratory mice. However, even though your supervisor enjoys your enthusiastic attitude, they do not allow you to do this quite yet because all you have is this plasmid. Why doesn’t your supervisor allow you to use the laboratory mouse for this research regarding animal welfare guidelines? Your answer should include the 3 R’s of…
One of the reasons why phage therapy has not been applied widely is that bacteria can become resistant to bacteriophages as well, through mutations in genes encoding for specific proteins. What would be a protein in the bacterial cell that, if mutated, would make that cell resistant to phage infection?
In the following "gene library" cloning experiment Digested genomic DNA AmpR gene TCR gene TCR is tetracycline resistant marker, AmpR is ampicillin resistant marker and BamHI is the unique restriction enzyme on plasmid. A PhD student digests/cuts the plasmids with BamHI restriction enzyme and the genomic DNA with EcoRI restriction enzyme. After performing the cloning experiment and obtaining colonies on a selection plate, the obtained cells will be ..... (Hint: this question is even more challenging; the PhD student was later demoted to an MSc student). a) resistant to ampicillin and tetracycline b) sensitive to tetracycline and ampicillin c) resistant to tetracycline and sensitive to ampicillin d) resistant to ampicillin and sensitive to tetracycline e) sensitive to ampicillin and tetracycline BamHI
Agrobacterium tumefaciens is a bacterium that infects a wide range of broad-leaved plants. During infection, a plasmid called Ti is transferred into the plant cells, where that plasmid DNA is integrated into the plant’s genome. Some of the genes encoded by this plasmid are expressed by host cell machinery in the plant cell. These gene products produce plant hormones which stimulate cell division in the infected cells, causing the development of a plant tumor which provides an environment for that bacterium to grow. You are studying a novel isolate of A. tumefaciens and want to determine which of the genes on the Ti plasmid are expressed in the bacterium, and which are expressed in the plant. You start by sequencing the Ti plasmid. a) Considering the DNA sequence of Ti, describe two specific genetic features that would help you to differentiate whether a given gene is likely expressed in the plant or bacterium. b) You collect plant tumor tissue, containing both bacterial and plant…
Describe how P1vir transduction can be used to introduce a gene mutation into E. coli USING DIAGRAMS ONLY. small figures to explain the diagrams is allowed. You should describe / show both the molecular biology and the transduction procedure
You are a graduate student working to construct a single gene knockout library of Leptospiria kirschneri, one the causative agents of leptospirosis. You are looking for single gene mutants which disrupt the bacterium’s spirillum shape to determine what role this rare cellular morphology may play in disease development and progression. Using an appropriate donor strain, you introduce the plasmid shown into L. kirschneri. L. kirschneri is not able to replicate the plasmid. The repeat regions are denoted on the plasmid map as vertical black lines, the transposase is denoted as tnp, and kanamycin kinase is denoted as aph. The larger of the two regions is transposed. Following selection and counter-selection, you isolate several non-spirillum colonies, which you use to infect juvenile piglets. Most of the infected piglets develop leptospirosis. Isolating L. kirschneri from these animals reveals that it has regained its spirillum morphology. What is a likely explanation for this reversion of…
Place the steps in the correct order from start to finish. Do not overlap steps. Start Transformed plant cells are selected for on a culture medium containing herbicide Bacteria are transformed with two plasmids Transgenes are incorporated into the plant cell genome T-DNA is transferred from the bacterial cell to the plant cell Transformed bacteria conjugate with plant cells Reset Transformed plant cells grow into transgenic plants Help Finish

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