E. coli cells are simultaneously infected with two strains of phage λ. One strain has a mutant host range, is temperature sensitive, and produces clear plaques (genotype h st c); another strain carries the wildtype alleles (genotype h+ st+ c+). Progeny phages are collected from the lysed cells and are plated on bacteria. The following numbers of different progeny phages are obtained: Progeny phage genotype Number of plaques h+ c+ st+ 321 h c st 338 h+ c st 26 h c+ st+ 30 h+ c st+ 106 h c+ st 110 h+ c+ st 5 h c st+ 6 a. Determine the order of the three genes on the phage chromosome. b. Determine the map distances between the genes. c. Determine the coefficient of coincidence and the interference
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E. coli cells are simultaneously infected with two strains of phage λ.
One strain has a mutant host range, is temperature sensitive, and
produces clear plaques (genotype h st c); another strain carries the
wildtype alleles (genotype h+ st+ c+). Progeny phages are collected
from the lysed cells and are plated on bacteria. The following
numbers of different progeny phages are obtained:
Progeny phage
genotype
Number of
plaques
h+ c+ st+ 321
h c st 338
h+ c st 26
h c+ st+ 30
h+ c st+ 106
h c+ st 110
h+ c+ st 5
h c st+ 6
a. Determine the order of the three genes on the phage
chromosome.
b. Determine the map distances between the genes.
c. Determine the coefficient of coincidence and the interference
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