As mentioned in question 2 of More Genetic TIPS, origins of transfer can be located in many different places on a bacterial chromosome, and their direction of transfer can be clockwise or counterclockwise. Let’s suppose a researcher conjugated six different Hfr strains that were thr + leu + tons str r azis lac + gal + pro + met + to an F − strain that was thr − leu − tonr str s azir lac − gal − pro − met −, and obtained the following results: Draw a map of the circular E. coli chromosome that shows the locations and orientations of the origins of transfer in these six Hfr strains.
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
As mentioned in question 2 of More Genetic TIPS, origins of
transfer can be located in many different places on a bacterial chromosome,
and their direction of transfer can be clockwise or counterclockwise.
Let’s suppose a researcher conjugated six different
Hfr strains that were thr + leu + tons str r azis lac + gal + pro + met +
to an F − strain that was thr − leu − tonr str s azir lac − gal − pro −
met −, and obtained the following results:
Draw a map of the circular E. coli chromosome that shows the
locations and orientations of the origins of transfer in these six Hfr
strains.
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