Suppose you wanted to study genes controlling thestructure of bacterial cell surfaces. You decide tostart by isolating bacterial mutants resistant to infection by a bacteriophage that binds to the cellsurface. The selection procedure is simple: Spreadcells from a culture of sensitive bacteria on a petriplate, expose them to a high concentration ofphages, and pick the bacterial colonies that grow.To set up the selection you could (1) spread cellsfrom a single liquid culture of sensitive bacteria onmany different plates and pick every resistant colony; or (2) start many different cultures, eachgrown from a single colony of sensitive bacteria,spread one plate from each culture, and then pick asingle mutant from each plate. Which methodwould ensure that you are isolating many independent mutations?

Q: Suppose that you I could obtain radioactively labeled penicillin. How could you use this compound to…

A: The penicillin works by preventing the production of peptidoglycan, which is a crucial component of…

Q: When a temperate phage infects a bacteria cell it O immediately undergoes the temperate lifecycle. O…

A: Phages can be either virulent or temperate. Lytic cycle is used for replication by virulent phage.…

Q: Experimentally, when an E. coli bacterium already has a λ prophageintegrated into its chromosome,…

A: Lambda phage binds to the bacterial cell surface and then, injects its genetic material inside the…

Q: Pegard to bacteriophage lambda's decision to enter the lytic or lysogenic cycle, which of following…

A: INTRODUCTION A bacteriophage, or phage for brief , is a virus that infects bacteria. Like other…

Q: The protein of T2 bacteriophage is labeled with 35S and the DNA with 32p. Where would one detect…

A: Introduction: An example of a virus that affects bacteria is the T2 bacteriophage. It belongs to the…

Q: Explain in molecular terms how a bacterial cell is made lysogenic by a temperate phage such a lambda

A: Introduction :- Lysogeny is the process in which a temperate phage, such as lambda, infects a…

Q: You have found that two types of bacteria (one large, one small) can undergo horizontal gene…

A: Bacteria are small single cell organisms. They found everywhere on earth. Some of them can survive…

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A: Surarnin is a potent and selective inhibitor of the RecA protein in Mycobacterium tuberculosis (Mtb)…

Q: Conjugation depends on direct cell-to-cell contactbetween a donor F+ carrying either a…

A: Conjugation is the process of genetic material transfer between two bacterial cells through direct…

Q: In Figure 5-12,a. Which Hfr gene enters the recipient last? (Whichdiagram shows it actually…

A: Some bacteria will have F-plasmid integrated with the chromosomal DNA, they are known as…

Q: Genome sequences show that some pathogenic bacteriacontain virulence genes that promote disease next…

A: Lateral gene transfer (also called horizontal gene transfer) has played an important role in…

Q: Before the integration of a transposon, its transposasemakes a staggered cut in the host target DNA.…

A: Introduction Deoxyribonucleic acid (DNA) is a polymer made up of two polynucleotide chains that coil…

Q: One of the frist steps in isolating plasmid DNA via mini-prep is to pellet the cells after O/N…

A: 1) The use of Resuspension buffer in the extraction or the isolation of the plasmid from the DNA is…

Q: When bacteriophage P1 causes E. coli to lyse, the resulting materialis called a P1 lysate. What type…

A: Transduction is the process by which a virus transfers genetic material from one bacterium to…

Q: E. coli cells are simultaneously infected with two strains of phage λ. One strain has a mutant host…

A: A gene is a stretch of nucleotides present in the DNA molecule. It encodes information for the…

Q: . In two isolates (one is resistant to ampicillin and theother is sensitive to ampicillin) of a new…

A: Bacteria are microscopic single-celled organisms that thrive in diverse environmental conditions.…

Q: The surface proteins on the lipid envelope of the hepatitis B virus specifically bind to receptor…

A: Introduction :- The virus known as HIV (human immunodeficiency virus) targets the immune system of…

Q: Cell line A was cultured in the absence and presence of KB9520 or PPT. You then lyse the cells and…

A: Estrogen receptors (ERs) are a family of transcription factors that play a critical role in a…

Q: You have constructed a cDNA library in a phagemid vector. Describe how you would screen the library…

A: Phagemid vectors are plasmid DNA [Deoxyribonucleic acid] containing a small portion of the genome of…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.