Tracking time of marker entry generates a chromosome map (a) 100 azi ton lac" gal 10 20 30 40 50 60 Time (minutes) (b) F factor 10 min Hir st Origin Origin Fstr 17 min 25 min FIGURE 5-12 in this intemupted mating conjugation experiment, F streptomycin-resistant cels with mutations in azi, ton, lac, and gal are incubated for varying times with Ht cels that are sensitive to streptomycin and cay wild-type aleles for these genes. (a) A plot of the frequency of donor aleles in exconjugants as a function of time after mating. (b) A schematic view of the transfer of markers (shown in diferent colors) with the passage of time. a Data trom EL Wolman, Facob, and W. Hayes, Cokd Sping Hartor Symp. Quant. Bol. 21, 1956, 141) charaders among str' excorjugants
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
In Figure 5-12,
a. Which Hfr gene enters the recipient last? (Which
diagram shows it actually entering?)
b. What is the maximum percentage of cases of transfer
of this gene?
c. Which genes have entered at 25 minutes? Could
they all become part of a stable exconjugant genome?
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
