You run a Western blot to detect Ras in sampe A and sample B. You find that sample B gives a darker band. What is the most appropriate interpretation of this? (a) sample A has more Ras DNA, (b) sample B has more Ras DNA, (c) sample A has more Ras mRNA, (d) sample B has more Ras mRNA, (e) sample A has more Ras protein, (f) sample B has more Ras protein. I understand that those molecules have higher molecular weight should give darker band. As mRNA has higher molecular weight than that of DNA and protein, I wish to pick (d) "sample B has more Ras mRNA" as the best answer. I am not sure if I am right. Please the expert advise.
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
You run a Western blot to detect Ras in sampe A and sample B. You find that sample B gives a darker band. What is the most appropriate interpretation of this? (a) sample A has more Ras DNA, (b) sample B has more Ras DNA, (c) sample A has more Ras mRNA, (d) sample B has more Ras mRNA, (e) sample A has more Ras protein, (f) sample B has more Ras protein.
I understand that those molecules have higher molecular weight should give darker band. As mRNA has higher molecular weight than that of DNA and protein, I wish to pick (d) "sample B has more Ras mRNA" as the best answer. I am not sure if I am right. Please the expert advise.
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Thank you. The expert seems made a typo. The correct answer should be "(f) sample B has more Ras protein", instead of (d).
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