(Construct 1-4) below could you use to make your own mouse knockout? For each one you did not choose, explain why you did not choose that construct. Part of DHCR7 gene structure is shown on top row in the schematic. (Antibiotic selection and negative selection genes have been abbreviated.) Gene DHCR7 Exoni Intron 1 Exon 2 Intron 2 Exon 3 Construct #1 Construct #2 Introni Negative Sel. Intron2 Antibio. Sel Introni Intron 2 Antibio. Sel. Negative Sel. Construct #3 Construct N4 Antibio. Sel Negative Sel. Antibio. Sel. Intron1 Intron2 Negative Sel. Introni Intron2

(Construct 1-4) below could you use to make your own mouse knockout? For each one you did not choose, explain why you did not choose that construct. Part of DHCR7 gene structure is shown on top row in the schematic. (Antibiotic selection and negative selection genes have been abbreviated.) Gene DHCR7 Exoni Intron 1 Exon 2 Intron 2 Exon 3 Construct #1 Construct #2 Introni Negative Sel. Intron2 Antibio. Sel Introni Intron 2 Antibio. Sel. Negative Sel. Construct #3 Construct N4 Antibio. Sel Negative Sel. Antibio. Sel. Intron1 Intron2 Negative Sel. Introni Intron2

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 20QP: Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant...

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Question

Which construct would you use to make a mouse model ?

(Construct 1-4) below could you use to make
your own mouse knockout? For each one
you did not choose, explain why you did not
choose that construct. Part of DHCR7 gene
structure is shown on top row in the
schematic. (Antibiotic selection and negative
selection genes have been abbreviated.)
Gene DHCR7
Exoni
Intron 1
Exon 2
Intron 2
Exon 3
Construct #1
Construct #2
Intron1
Negative Sel.
Intron2
Antibio. Sel
Intron1
Intron 2
Antibio. Sel. Negative Sel.
Construct #3
Construct #4
Antibio. Sel Negative Sel.
Antibio. Sel.
Introni
Intron2
Negative Sel.
Intron1
Intron2

Expert Solution

Hello

The selected DHRC should follow the this procedure -

1.Use of electroporation -

2. Homologous recombination for knocked-out gene into their chromosoms in place of the original gene.

2. Vector use for transformed with neomycin resistance gene via homologous recombination.

3. Intron 1= random insertion will die because of positive test for both the neomycin resistance gene.

4 . Intron 2= for giving resistivity for wanted resistance.

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