(Construct 1-4) below could you use to make your own mouse knockout? For each one you did not choose, explain why you did not choose that construct. Part of DHCR7 gene structure is shown on top row in the schematic. (Antibiotic selection and negative selection genes have been abbreviated.) Gene DHCR7 Exoni Intron 1 Exon 2 Intron 2 Exon 3 Construct #1 Construct #2 Introni Negative Sel. Intron2 Antibio. Sel Introni Intron 2 Antibio. Sel. Negative Sel. Construct #3 Construct N4 Antibio. Sel Negative Sel. Antibio. Sel. Intron1 Intron2 Negative Sel. Introni Intron2

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Which construct would you use to make a mouse model ?
(Construct 1-4) below could you use to make
your own mouse knockout? For each one
you did not choose, explain why you did not
choose that construct. Part of DHCR7 gene
structure is shown on top row in the
schematic. (Antibiotic selection and negative
selection genes have been abbreviated.)
Gene DHCR7
Exoni
Intron 1
Exon 2
Intron 2
Exon 3
Construct #1
Construct #2
Intron1
Negative Sel.
Intron2
Antibio. Sel
Intron1
Intron 2
Antibio. Sel. Negative Sel.
Construct #3
Construct #4
Antibio. Sel Negative Sel.
Antibio. Sel.
Introni
Intron2
Negative Sel.
Intron1
Intron2
Transcribed Image Text:(Construct 1-4) below could you use to make your own mouse knockout? For each one you did not choose, explain why you did not choose that construct. Part of DHCR7 gene structure is shown on top row in the schematic. (Antibiotic selection and negative selection genes have been abbreviated.) Gene DHCR7 Exoni Intron 1 Exon 2 Intron 2 Exon 3 Construct #1 Construct #2 Intron1 Negative Sel. Intron2 Antibio. Sel Intron1 Intron 2 Antibio. Sel. Negative Sel. Construct #3 Construct #4 Antibio. Sel Negative Sel. Antibio. Sel. Introni Intron2 Negative Sel. Intron1 Intron2
Expert Solution
Hello

The selected DHRC should follow the this procedure -

1.Use of electroporation -

2.  Homologous recombination for knocked-out gene into their chromosoms in place of the original gene.

2. Vector use for transformed with neomycin resistance gene via homologous recombination.

3. Intron 1=   random insertion will die because of  positive test for both the neomycin resistance gene.

4 . Intron 2= for giving resistivity for wanted resistance.

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