After decades of work, Dr. Ricky M. isolated a small amount of attractase—an enzyme that produces a powerful human pheromone—from hair samples of Hollywood celebrities. to take advantage of attractase for his personal use, he obtained a complete genomic clone of the attractase gene, connected it to a strong bacterial promoter on an expression plasmid, and introduced the plasmid into E. coli cells. He was devastated to find that no attractase was produced in the cells. What is a likely explanation for his failure?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
After decades of work, Dr. Ricky M. isolated a small amount of attractase—an enzyme that produces a powerful human pheromone—from hair samples of Hollywood celebrities. to take advantage of attractase for his personal use, he obtained a complete genomic clone of the attractase gene, connected it to a strong bacterial promoter on an expression plasmid, and introduced the plasmid into E. coli cells. He was devastated to find that no attractase was produced in the cells. What is a likely explanation for his failure?
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