Antibiotic-resistance is one of the gravest dangers to the world’s medical system. As a result, Dr. Jhon Peῆa decides to apply for a research grant from the NIH to investigate novel targets for antibiotic treatment in his lab. Most bacteria have developed resistance to counter the effects of the current crop of antibiotics, which largely target the cell wall, plasma membrane, or ribosomes. Dr. Peῆa’s team has instead been investigating bacterial transcriptional sites and all of his students are asked to attend a group Zoom meeting to discuss ideas to put into the grant. 1) Dr. Wilhem, his post-doc, mentions that heat-sensitive RNA pol mutants found in yeast might be worth exploring to see if they could be genetically engineered into E. coli. He reckons that anyone who is sick from a bacterial infection typically develops a fever so this would activate their destruction. a GREAT IDEA! b SUCKY IDEA! Yeast are eukaryotic so their RNA pol binds to a different site from that of E. coli. c Yeast are durable organisms so one would need to figure out at what temperature the RNA pol deactivated to make sure it would be comparable to the temperature a human would produce from a bacterial infection. d This wouldn’t help destroy wildtype pathogenic bacteria since they won’t contain the same defective heat sensitive eukaryotic alleles of RNA pol that you plan to introduce into genetically engineered bacteria. 2) Jada, an undergrad student doing a lab tutorial with Dr. Peῆa, suggests keeping it simple; screen chemicals that can bind to an allosteric site on sigma factor to prevent it from being able to subsequently bind to TATAAT, which would block transcription. What do the other graduate students think? a Guess we’re not going to get that grant!?! b There is a reason that undergrads are not allowed to do doctoral work! c She doesn’t even have her Bachelor’s yet, hello??! d This would make sense if there exists a model of sigma factor protein to investigate for allosteric binding sites.

Antibiotic-resistance is one of the gravest dangers to the world’s medical system. As a result, Dr. Jhon Peῆa decides to apply for a research grant from the NIH to investigate novel targets for antibiotic treatment in his lab. Most bacteria have developed resistance to counter the effects of the current crop of antibiotics, which largely target the cell wall, plasma membrane, or ribosomes. Dr. Peῆa’s team has instead been investigating bacterial transcriptional sites and all of his students are asked to attend a group Zoom meeting to discuss ideas to put into the grant. 1) Dr. Wilhem, his post-doc, mentions that heat-sensitive RNA pol mutants found in yeast might be worth exploring to see if they could be genetically engineered into E. coli. He reckons that anyone who is sick from a bacterial infection typically develops a fever so this would activate their destruction. a GREAT IDEA! b SUCKY IDEA! Yeast are eukaryotic so their RNA pol binds to a different site from that of E. coli. c Yeast are durable organisms so one would need to figure out at what temperature the RNA pol deactivated to make sure it would be comparable to the temperature a human would produce from a bacterial infection. d This wouldn’t help destroy wildtype pathogenic bacteria since they won’t contain the same defective heat sensitive eukaryotic alleles of RNA pol that you plan to introduce into genetically engineered bacteria. 2) Jada, an undergrad student doing a lab tutorial with Dr. Peῆa, suggests keeping it simple; screen chemicals that can bind to an allosteric site on sigma factor to prevent it from being able to subsequently bind to TATAAT, which would block transcription. What do the other graduate students think? a Guess we’re not going to get that grant!?! b There is a reason that undergrads are not allowed to do doctoral work! c She doesn’t even have her Bachelor’s yet, hello??! d This would make sense if there exists a model of sigma factor protein to investigate for allosteric binding sites.

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Description: Antibiotic-resistance is one of the gravest dangers to the world’s medical system. As a result, Dr. Jhon Peῆa decides to apply for a research grant from the NIH to investigate novel targets for antibiotic treatment in his lab. Most bacteria have deve

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Antibiotic-resistance is one of the gravest dangers to the world’s medical system. As a result, Dr. Jhon Peῆa decides to apply for a research grant from the NIH to investigate novel targets for antibiotic treatment in his lab. Most bacteria have developed resistance to counter the effects of the current crop of antibiotics, which largely target the cell wall, plasma membrane, or ribosomes. Dr. Peῆa’s team has instead been investigating bacterial transcriptional sites and all of his students are asked to attend a group Zoom meeting to discuss ideas to put into the grant.

Dr. Wilhem, his post-doc, mentions that heat-sensitive RNA pol mutants found in yeast might be worth exploring to see if they could be genetically engineered into E. coli. He reckons that anyone who is sick from a bacterial infection typically develops a fever so this would activate their destruction.

GREAT IDEA!

SUCKY IDEA! Yeast are eukaryotic so their RNA pol binds to a different site from that of E. coli.

Yeast are durable organisms so one would need to figure out at what temperature the RNA pol deactivated to make sure it would be comparable to the temperature a human would produce from a bacterial infection.

This wouldn’t help destroy wildtype pathogenic bacteria since they won’t contain the same defective heat sensitive eukaryotic alleles of RNA pol that you plan to introduce into genetically engineered bacteria.

Jada, an undergrad student doing a lab tutorial with Dr. Peῆa, suggests keeping it simple; screen chemicals that can bind to an allosteric site on sigma factor to prevent it from being able to subsequently bind to TATAAT, which would block transcription. What do the other graduate students think?

Guess we’re not going to get that grant!?!

There is a reason that undergrads are not allowed to do doctoral work!

She doesn’t even have her Bachelor’s yet, hello??!

This would make sense if there exists a model of sigma factor protein to investigate for allosteric binding sites.

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