e following genetic and cellular conditions. You do not need to consider the role of CAP in your analysis. Genes Repressed (yes/no) Constitutive (yes/no) IS O+ Z+
Q: If the above gene is one of the three structural genes of the lac operon that codes for the protein/…
A: The bacterium Ecoli possess a highly effective lactose Metabolism process . The lac operon is a…
Q: Under which conditions does the trp repressor block transcription of the trp operon? when the…
A: The trp operon responds to a repressor protein that binds to two molecules of tryptophan. When the…
Q: A mutation at the operator prevents the regulator protein from binding. What effect will this…
A: An operon includes a group of genes that have a common promoter, regulator, and would be transcribes…
Q: A bacterial operon consists of _____________________________________________ a single gene that…
A: Bacterial operons are coregulated gene clusters.These genes are turned on or off together.This…
Q: Operon X produces three enzymes that convert Compound A to Compound B. The R gene makes the…
A: Gene expression is given on the basis of RNA. It is a process through which there is turning on of…
Q: What effect will the above process have on the tryptophan operon? RNA polymerase will detach from…
A: An operon is a functional unit of DNA (Deoxyribonucleic acid) containing a cluster of genes under…
Q: In the lac operon of E. coli, if glucose levels increase while lactose levels decrease, what will…
A: Gene regulation at the level of transcription in bacteria is achieved by the operon model. Operon…
Q: A new operon called Terperon is discovered and is not normally transcribed. It is stimulated by the…
A: Operon is the cluster of regulatory, promoter, operator and structural genes
Q: The trp operon in E. coli encodes enzymes essential for the biosynthesis of tryptophan. In the…
A: trp operon is a repressor operon found in E. coli bacteria, which consists of a group of genes that…
Q: n the tryptophan operon of E. coli, what happens when tryptophan levels get too high? tryptophan is…
A: An operon is a functional unit of DNA (Deoxyribonucleic acid) containing a cluster of genes under…
Q: Listed in parts a through g are some mutations that were found in the 5′ UTR of the trp operon of E.…
A: Since we only answer up to 3 sub-parts, we’ll answer the first 3. Please resubmit the question and…
Q: When cAMP binds to the Catabolite Activator Protein (CAP), it causes a conformational change on the…
A: The lac operon will be expressed at high levels if the following two criteria are met: Glucose must…
Q: A mutation that inactivates transcription and translation from the regulatory gene of an inducible…
A: An operon is a functional unit of genomic DNA that comprises a collection of genes that are all…
Q: A mutation in the lac I gene that prevents production of the lac repressor choose an answer below:…
A: lac operon also known as lactose operon was discovered by F. Jacob and J. Monod, for that, they…
Q: Which of the following mutations would result in the highest level of lac operon transcription in…
A:
Q: What are the effects of the following conditions on Lac operon of bacteria? Do not forget to mention…
A: Lac Operon helps in the metabolism of lactose in bacteria.
Q: Glucose absent, lactose absent Glucose absent, lactose present Glucose present, lactose…
A: An operon is found in bacteria and viruses as a genetic regulatory system. The gene coding for…
Q: An E. coli cell acquires a mutation in the gene that encodes CAP. The mutation affects the…
A: The transcription is a process through which information in the DNA molecule is transcribed into…
Q: You are studying the ABCD operon from a newly isolated bacterium. Which of the following would…
A: A Operon consists of segments of DNA , which carry codes for the synthesis of proteins. These genes…
Q: E. coli has five genes that code for enzymes that make tryptophan. These genes are regulated by a…
A: Tryptophan operon found in E.coli and some other bacteria. It is group of genes which encodes for…
Q: Expression of the operon ultimately results in the synthesis of the repressor (Q) and co-repressor…
A: A repressor is a protein that turns off the expression of one or more genes. The repressor protein…
Q: The lac operon consists of three structural genes, lacZ, lacY and lacA that are transcribed as a…
A:
Q: Which of the following describes the lac operon in E. coli when lactose and glucose is present in…
A: The lac repressor acts as a lactose sensor. It normally blocks transcription of the operon, but…
Q: As diagrammed below, some amino acid biosynthetic operons have stem loop structures in the mRNA.…
A: Option A is incorrect as without the formation of heparin the process of translation can not be…
Q: What are the effects of the following conditions on Lac operon of bacteria? Do not forget to mention…
A: lac operon: - inducible operon. - Both positive and negative regulation. - Involved in lactose…
Q: All the following are characteristics of inducible operons except they are normally inactive they…
A: Inducible operons are catabolic pathways as the enzymes involved in catabolism do not need to be…
Q: Like the lac operon, the hexose operon is controlled by a separate regulatory protein under the…
A: Hexose operon is the set of structural genes, regulatory genes, promoter and operator sequence in…
Q: The regulation of the lac operon in E. coli by Catabolite Activator Protein and glucose is an…
A: An operon is essentially a gene regulatory system in which a collection of genes coding for…
Q: For the lac operon, when glucose is absent and lactose is present ___________ None of the…
A: Question - For the lac operon, when glucose is absent and lactose is present ___________…
Q: Consider the trp operon found in E. coli. You have discovered a mutant strain of E.coli that…
A: The genome of the prokaryotic organisms is polycistronic i.e. the genes are controlled by one…
Q: If E. coli bacteria are placed in an environment that contains both glucose and lactose, the…
A: operon are made up of various structural gene which are regulated by same promoter and operator.
Q: What is the role of catabolite activator protein (CAP) in the lactose operon of E. coli? it…
A: Catabolite activator protein is a transcriptional activator. It binds RNA polymerase and activates…
Q: What is the function of cAMP in regulation of the lac operon? Group of answer choices it activates a…
A: The lac operon seems to be an inducible operon that uses lactose as a source of energy and is active…
Q: What is the role of catabolite activator protein (CAP) in the lactose operon of E. coli?
A: Lac operon Lac operon is a group of protein that code for enzyme β-galactosidase. The function of…
Q: Positive repressible means the regulatory protein is a(n) ______, and the operon is ______ when the…
A: In the case of the positive repressible operon, the activator protein is found to be bound and…
Q: Like the lac operon, the hexose operon is controlled by a separate regulatory protein under the…
A: Hexose operon is the segment of DNA which includes regulatory gene, structural gene, promoter and…
Q: What is a specific region in the trp operon that you could mutate so it expresses the trp operon in…
A: Introduction : The Trp Operon Codes For The Components Required For Tryptophan Production. An Operon…
Q: The operator of the lactose operon in E. Coli is located: between the promoter and the…
A: The lac operon in Ecoli is concerned with the digestion process of the lactose sugar. For this gene…
Q: Theara operon is an inducible operon that controls the production of the sugar arabinose. When…
A: An operon is a group of genes that are transcribed together and are under the influence of a single…
Q: Like the lac operon, the hexose operon is controlled by a separate regulatory protein under the…
A: The operons are regions in DNA which have a cluster of related genes and these function as a unit.…
Q: A mutation occurs in the 5′ UTR of the trp operon that reduces the ability of region 2 to pair with…
A: In the absence of tryptophan, the trp operon would be expressed maximum as the trp repressor would…
Q: The lac operon is a negative inducible system and allolactose will bind to the regulator protein…
A: In normal state, the lac operon is generally turned off. A repressor protein during "off" stage,…
Q: In the tryptophan operon of E. coli, what happens when tryptophan levels get too high? tryptophan…
A: A trp operon is a group of genes that are transcribed, together to code for the components for the…
Q: The lac operon produces enzymes that allow the bacteria E. coli to metabolize lactose, it is in an…
A: Operon is a bacterial and viral genetic regulation mechanism in which genes coding for functionally…
Q: For each of the following types of transcriptional control, indicate whether the protein produced by…
A: An inducible operon is one which is not transcribed. For transcription of the molecule, It requires…
Q: In the lac operon, the lacZ gene codes for an enzyme called ____________, which catalyze the…
A: 1st blank: beta-galactosidase 2nd blank: Lactose
Q: Suppose that E. coli sustains a mutation in its gene for the lac operon repressor making the…
A: The lactose operon or lac operon is an inducible operon system under dual – positive and negative…
Q: The blob operon produces enzymes that convert compound A into compound B. The operon is controlled…
A: Operon is a term used for the functional unit of the DNA that contains the cluster of genes which…
Q: The figure above shows the structure of lac operon. Which of the following BEST describes what…
A: These are the operon gene found in prokaryotes and the lac operon is an inducible operon.
The lac operon of E. coli controls the expression of genes that code for enzymes involved in lactose
I - = produces a repressor protein that cannot bind to the operator region
IS = produces a repressor protein that cannot be removed from the operator region
OC = cannot bind a repressor protein
Z - = produces a defective (non-functioning) β-galactosidase enzyme
+ = wild type
Detail the functionality of the lac operon under the following genetic and cellular conditions. You do not need to consider the role of CAP in your analysis.
Genes |
Repressed (yes/no) |
Constitutive (yes/no) |
IS O+ Z+
|
|
|
IS OC Z+
|
|
|
Step by step
Solved in 4 steps
- A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcriptionThe lac operon of E. coli controls the expression of genes that code for enzymes involved in lactose metabolism. Mutations to various regions of the lac operon have been discovered which affect both the control of gene expression and the function of the gene products. These mutations include: I - = produces a repressor protein that cannot bind to the operator region IS = produces a repressor protein that cannot be removed from the operator region OC = cannot bind a repressor protein Z - = produces a defective (non-functioning) β-galactosidase enzyme + = wild type Detail the functionality of the lac operon under the following genetic and cellular conditions. You do not need to consider the role of CAP in your analysis. Genes Repressed (yes/no) Constitutive (yes/no) I+ OC Z+ I+ O+ Z+ (Lactose present) I- O+ Z+Given the following mutant and conditions, predict the expression of the lacZ gene (no expression, basal level, activate level of expression). A mutant of E. coli that has a mutation in the operator of the lac operon that prevents the repressor from binding. + glucose, - lactose + glucose, + lactose – glucose, + lactose
- Given the following mutant and conditions, predict the expression of the lacZ gene (no expression, basal level, activate level of expression). A mutant of E. coli has a mutation in the promoter of the lac operon that prevents RNA polymerase from binding. + glucose, - lactose + glucose, + lactose – glucose, + lactoseGiven the Table below concerning the lac operon, match the letter with what would be occurring for each scenario. Repressor is bound to the operator, cAMP complex is bound and transcription is prevented. Allolactose is bound to the repressor, cAMP complex is bound, and transcription is prevalent. Allolactose is bound to the repressor, cAMP complex is not assembled, and transcription is slow Repressor is bound to the operator, cAMP complex is not assembled, and transcription is prevented.In the tryptophan operon of E. coli, what happens when tryptophan levels get too high? tryptophan is removed from its co-repressor, causing it to detach from the operator tryptophan is removed from its co-repressor, causing it to attach to the operator tryptophan is bound to its co-repressor, causing it to detach from the operator tryptophan is bound to its co-repressor, causing it to attach to the operator all of the above occur at equal rates when tryptophan levels get too high (Option C is the answer for the question above) What effect will the above process have on the tryptophan operon? RNA polymerase will detach from the promoter, initiating transcription RNA polymerase will detach from the promoter, stopping transcription RNA polymerase will attach to the promoter, initiating transcription RNA polymerase will attach to the promoter, stopping transcription all of the above occur at equal rates when tryptophan levels get too high
- In the tryptophan operon of E. coli, what happens when tryptophan levels get too high? tryptophan is removed from its co-repressor, causing it to detach from the operator tryptophan is removed from its co-repressor, causing it to attach to the operator tryptophan is bound to its co-repressor, causing it to detach from the operator tryptophan is bound to its co-repressor, causing it to attach to the operator all of the above occur at equal rates when tryptophan levels get too high (Option D is the answer for the question above) What effect will the above process have on the tryptophan operon? RNA polymerase will detach from the promoter, initiating transcription RNA polymerase will detach from the promoter, stopping transcription RNA polymerase will attach to the promoter, initiating transcription RNA polymerase will attach to the promoter, stopping transcription all of the above occur at equal rates when tryptophan levels get too highIn the tryptophan operon of E. coli, what happens when tryptophan levels get too high? tryptophan is removed from its co-repressor, causing it to detach from the operator tryptophan is removed from its co-repressor, causing it to attach to the operator tryptophan is bound to its co-repressor, causing it to detach from the operator tryptophan is bound to its co-repressor, causing it to attach to the operator all of the above occur at equal rates when tryptophan levels get too high What effect will the above process have on the tryptophan operon? RNA polymerase will detach from the promoter, initiating transcription RNA polymerase will detach from the promoter, stopping transcription RNA polymerase will attach to the promoter, initiating transcription RNA polymerase will attach to the promoter, stopping transcription all of the above occur at equal rates when tryptophan levels get too highThe trp operon in E. coli encodes enzymes essential for the biosynthesis of tryptophan. In the operon, trpR gene encodes the repressor. A trpR repressor cannot bind tryptophan. The trpO is the operator. trpA encodes the enzyme tryptophan synthetase. For the enzyme (A), indicate with a + or-whether or not it is made when there is tryptophan and when there is no tryptophan. Without Tryptophan With Tryptophan R'O A /R* O' A R'O' A/R* Oʻ A R*OA/R' O A
- Like the lac operon, the hexose operon is controlled by a separate regulatory protein under the control of its own promoter (see the schematic of the operon below). The hexose regulatory protein is sensitive to fatty acyl CoA levels. When all hexose fuel sources are depleted, the bacteria switch to lipid metabolism and fatty acyl CoA levels increase. This turns expression of the hexose operon off. +1 +1 Regulatory Gene P regulator Hexose Operon Genes operon regulator promoter operon promoter e. The regulatory protein that controls expression of the hexose operons is a transcriptional ACTIVTOR or REPRESSOR (circle one).The map of the lac operon is shown below. Consider the following examples that include both haploids and partial diploids and explain in each scenario whether the repressor can bind and regulate expression and whether or not the lac operon is expressed. For partial diploids the plasmid is indicated by the F’. I+ O+ Z+ Y+ / F’ I+ O+ Z+ Y+ I- O+ Z+ Y+ / F’ I+ O+ Z+ Y+ I- O+ Z+ Y+ I+ Oc Z+ Y+The trp operon is: **(for additional information review the CogBooks Module, answer explained on CogBooks): In the absence of tryptophan, the trp repressor dissociates from the operator, and RNA synthesis proceeds. promoter operator trpE| trpD trpC trpB trpA RNA polymerase repressor When tryptophan is present, the trp repressor binds the operator, and RNA synthesis is blocked. promoter operator trpE trpD trpC trpB trpA RNA polymerase; repressor tryptophan Both negative inducible and positive repressible Positive inducible Negative Repressible O Negative inducible