Examine the following theoretical operon (gen) in E. coli which contains structural genes (genC, genB, genA) that are involved in amino acid biosynthesis. The gen operon also includes the gene encoding for the regulator molecule within it. When the amino acid that this operon helps synthesize is present, it binds to the regulator forming a complex which can bind to the operator region. Assume that the regulator molecule only binds to the operator in the presence of the amino acid. Promoter || Operator genC genB gen operon Regulator genA Question 3 Assume each gene in the gen operon has its own start and stop codons. How would a nonsense mutation in the genB region influence the expression of the gen operon in the absence of the amino acid? A. Gain of function B. Loss of function C. Null D. Silent E. Insufficient information Question 3 Assume each gene in the gen operon has its own start and stop codons. How could a base substitution mutation in the operator region influence the expression of the gen operon in the abundance of the amino acid? A. Gain of function B. Loss of function C. Null D. Silent E. Insufficient information

Examine the following theoretical operon (gen) in E. coli which contains structural genes (genC, genB, genA) that are involved in amino acid biosynthesis. The gen operon also includes the gene encoding for the regulator molecule within it. When the amino acid that this operon helps synthesize is present, it binds to the regulator forming a complex which can bind to the operator region. Assume that the regulator molecule only binds to the operator in the presence of the amino acid. Promoter || Operator genC genB gen operon Regulator genA Question 3 Assume each gene in the gen operon has its own start and stop codons. How would a nonsense mutation in the genB region influence the expression of the gen operon in the absence of the amino acid? A. Gain of function B. Loss of function C. Null D. Silent E. Insufficient information Question 3 Assume each gene in the gen operon has its own start and stop codons. How could a base substitution mutation in the operator region influence the expression of the gen operon in the abundance of the amino acid? A. Gain of function B. Loss of function C. Null D. Silent E. Insufficient information

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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If a wild-type (normal, NOTmutated) E. coli strain is grown in a medium: a. without lactose or glucose, how many proteins (and which ones) are bound to the lac operon? b. Without lactose, but with glucose, how many proteins (and which ones) are bound to the lac operon??
WHAT ELSE CAN I ADD TO THIS DISCUSSION In general, what is gene regulationand what are the consequences if this process goes awry? Gene regulation is the process used to control the timing, location and amount of genes expressed. The process is carried out by a variety of mechanisms, including regulatory proteins and chemical modification of DNA. It’s key to the ability of an organism to respond to environmental changes. If the gene regulation process goes awry, these alterations are called mutations and can accumulate over a lifetime. Errors in genes that control cell division can cause cancer and other genetic mutations to take place.
Answer these two multiple choice questions
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PLEASE EXPLAIN EACH STEP OF THIS QUESTION
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You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation a.Which operons would be functional under these conditions? b.What repressors would be made? (NOTE: name the repressors using their gene names) c.Which repressor(s) would be made in the inactive form? d.Which repressor(s) would be made in the active form? e.Which repressor(s) under these conditions can bind the operator sequence? f.Which repressor(s)under these conditions cannot bind the operator sequence?