## Transcription of the Lac Operon in E. coli### Question:Consider a mutant of *E. coli* that has an inactivating mutation in the gene for adenylate cyclase (*cya*). Choose the compound(s) that could be provided to the mutant to achieve full transcription of the lac operon. If no combination of compounds can allow for transcription, choose **none**.### Options:- [ ] ATP- [x] cAMP- [ ] glucose- [ ] lactose- [ ] none### Explanation:Adenylate cyclase is an enzyme that catalyzes the conversion of ATP to cyclic AMP (cAMP). In *E. coli*, cAMP levels are crucial for the activation of the lac operon. The presence of cAMP, along with the absence of glucose and the presence of lactose, allows the transcription of the lac operon by binding to and activating the cAMP receptor protein (CRP), which in turn binds to the promoter region of the lac operon to facilitate RNA polymerase binding.- **ATP**: Providing ATP would not be sufficient because the mutation in adenylate cyclase prevents it from being converted to cAMP.- **cAMP**: Supplementing cAMP compensates for the defective adenylate cyclase enzyme, enabling the activation of the lac operon.- **glucose**: High levels of glucose inhibit the formation of cAMP, hindering the transcription of the lac operon.- **lactose**: Lactose is necessary to relieve the repression of the lac operon but alone cannot activate transcription without cAMP.- **none**: This option indicates that no combination can restore lac operon transcription, which is not correct in this case, as cAMP can enable full transcription.Thus, providing cAMP to the mutant strain allows full transcription of the lac operon despite the mutation in the adenylate cyclase gene.

Name: ## Transcription of the Lac Operon in E. coli### Question:Consider a
Uploaded: 2024-03-20T06:42:41.000Z
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Description: ## Transcription of the Lac Operon in E. coli### Question:Consider a mutant of *E. coli* that has an inactivating mutation in the gene for adenylate cyclase (*cya*). Choose the compound(s) that could be provided to the mutant to achieve full transcr