Need answer and explain You mutated the gene encoding the cyclic AMP receptor protein (CRP). In this mutant, the CRP isproduced but is unable to bind cyclic AMP. How will this mutant affect transcription of the lacoperon?O The lac operon will always be transcribed at a high rate.O The lac operon will only be transcribed at a high rate when lactose is present and glucose is not.O The lac operon will only be transcribed at a high rate when glucose is present and lactose is not.The lac operon will never be transcribed at a high rate.

Operon is a gene regulatory structure that contains clusters of genes and these clusters of genes…

You mutated the gene encoding the cyclic AMP receptor protein (CRP). In this mutant, the CRP is produced but is unable to bind cyclic AMP. How will this mutant affect transcription of the lac operon? O The lac operon will always be transcribed at a high rate. The lac operon will only be transcribed at a high rate when lactose is present and glucose is not. The lac operon will only be transcribed at a high rate when glucose is present and lactose is not. O The lac operon will never be transcribed at a high rate.

You mutated the gene encoding the cyclic AMP receptor protein (CRP). In this mutant, the CRP is produced but is unable to bind cyclic AMP. How will this mutant affect transcription of the lac operon? O The lac operon will always be transcribed at a high rate. The lac operon will only be transcribed at a high rate when lactose is present and glucose is not. The lac operon will only be transcribed at a high rate when glucose is present and lactose is not. O The lac operon will never be transcribed at a high rate.

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 9TYU: A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous...

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Match the nutrient environment with the status of the transcription factors that regulate the lac operon in E. coli. Lacl bound to lactose and CAP is bound V[Choose ] Low lactose, low glucose High lactose, high glucose High lactose, low glucose Low lactose, high glucose CAMP and the CAP-O Lacl bound to the LacO and CAP bound CAMP and the CAP-O Lacl bound to lactose and CAP unbound [ Choose ] by CAMP, not on the CAP-O Lacl bound to the LacO and CAP unbound [ Choose ] by CAMP, not on the CAP-O
Consider a mutant of E. coli that has an inactivating mutation in the gene for adenylate cyclase (cya). Choose the compound(s) that could be provided to the mutant to achieve full transcription of the lac operon. If no combination of compounds can allow for transcription, chose none. O ATP O CAMP O glucose O lactose none
Consider a mutant of E. coli that has an inactivating mutation in the gene for the catabolite activator protein (cap). Choose the compound(s) that could be provided to the mutant to achieve full transcription of the lac operon. If no combination of compounds can allow for transcription, chose none. ATP CAMP O glucose lactose none
High levels of glucose in the environment result in low levels of transcription of the lac operon. The reason for this is O glucose binds to the Lac Repressor to cause it to bind to the operator site (lac O) glucose lowers the levels of CAMP, which is necessary to activate the CAP protein O giucose raises the levels of CAMP, which binds to the CAP protein to cause transcription repression O glucose prevents lactose from binding to the Lac Repressor O glucose lowers the levels of the CAP protein, which is necessary to stimulate transcription of the lac operon
If glucose is not available, but lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
Mutations in the genes of the lac operon might affect the regulation of β-galactosidase synthesis. For each mutation listed below, indicating whether β-galactosidase would be regulated normally, always ON or always OFF. Mutation in operator site prevents repressor binding Mutation in lacIgene prevents repressor from binding operator Mutation in lacIgene prevents repressor from binding allolactose Nonsense mutation in lacZgenere
Under what conditions would you expect to see high levels of expression of the structural genes in the lac operon? Group of answer choices Lactose is absent and glucose is absent Lactose is absent and glucose is present Lactose is present and glucose is present None of these Lactose is present and glucose is absent
Given the Table below concerning the lac operon, match the letter with what would be occurring for each scenario. Repressor is bound to the operator, cAMP complex is bound and transcription is prevented. Allolactose is bound to the repressor, cAMP complex is bound, and transcription is prevalent. Allolactose is bound to the repressor, cAMP complex is not assembled, and transcription is slow Repressor is bound to the operator, cAMP complex is not assembled, and transcription is prevented.
One way that regulation is often studied is by examining the effects of mutations. For example, mutations within the parts of the lac operon have been well studied. Which of these mutants would be considered a constitutive mutant (the operon is always on)? a mutation in CAP that prevents the binding of CAMP a mutation in one of the structural genes that prevents the transport of lactose into the cell a mutation that disables the promoter for the operon a mutation that disables the repressor
As diagrammed below, some amino acid biosynthetic operons have stem loop structures in the mRNA. These structures play a role in the regulation of the operon by which of the following mechanisms? Translation stop codon- Attenuator loop. mRNA 5' 3' DNA attenuator trpE O Catabolite repressor protein can bind and prevent the hairpins from forming thus allowing translation to occur. O The leader sequence shown contains codons for the amino acid. When these are low, the ribosome stalls, allowing a hairpin between the two middle regions to form so that the rest of the message can be translated. In the presence of large amounts of the amino acid, the hairpin will wind around the amino acid and prevent further translation. O A repressor protein made of that amino acid will bind to the region and prevent translation when large amounts of the amino acid are present. O The uridine-rich region has codons for that amino acid; when present, this region will be a terminator region. The hairpins allow…
In the lac operon (below), how will expression of the genes lacZ and lacy be effect by a mutation in the repressor protein (encoded by lach) that prevents it from binding the operator sequence (lacO) in the absence of lactose? Explain the answer
To characterize the promoter of the gadA operon you made a series of deletion mutants removing pieces of the promoter to see what would happen. The results are found below: gad promoter gada gadX gadz 450 +1 lacz activity transcription start site pH 2.0 pH 7.0 A gad promoter beta-galactosidase (lacZ) +++ 450 gad promoter beta galactosidase (lacZ) +++ +++ 300 +1 gad promoter beta galactosidase (lacZ) 150 D gad beta galactosidase (lacz) -450 150 E gad promoter beta-galactosidase (lacZ) -450 -300 Based on these results, what can you conclude about the gad promoter? O a. The promoter is only regulated by repression Ob. The promoter is regulated by a mix of activation and repression O c. The promoter is only regulated by activation O d. The promoter has multiple operators and multiple enhancers