How would you use a ChIP (chromatin immunoprecipitation) to show that when RNA pol II is bound at the promoter of Gene XYZ it recruits Capping Enzyme (but not PolyA Polymerase), and that when RNA pol II is downstream of the promoter near the polyA signal for Gene XYZ that it recruits PolyA Polymerase (but not Capping Enzyme)? Be sure to show what positive results would look like and indicate what controls you would use. I am looking for a well developed plan with lots of details with an illustration of the ideal autoradiogram, with info on primers, antibodies, etc.

Q: Describe the difference between a transcriptional fusion and a translational reporter gene fusion.…

A: Transcription fusion means cloning the promoter of interest, upstream of any transcription unit,…

Q: SPO11 re

A:

Q: The tac promoter, an artificial promoter made from portions of the frp and lacUV5 promoters, has…

A: The lac operon is an operon required for the transport and metabolism of lactose in E.coli and many…

Q: Mediator is not necessary to reproduce transcription initiation reactions in vitro. If you are…

A: In the study of eukaryotic gene expression, understanding the process of transcription initiation is…

Q: Which of these pairs of clinical applications of DNA repair mechanisms is not accurate? Group of…

A: To correct the DNA damage within cells, the cell uses various cellular repair mechanisms called DNA…

Q: Mutation in the operator that reduces the affinity of the operator for the repressor protein…

A: Lac operon is an inducible operon. When lactose is present in medium, then lac operon starts.

Q: st noncoding RNA (ncRNA) triggers X inactivation by coating the X chromosome that produces it and by…

A: Xist is a long non-coding RNA on the X chromosome. Xist acronym is X -inactive-specific transcript…

Q: Complete Table 1, indicating how much (lots, little, none) of the above gene product would be made…

A: An operon is a single transcription unit that can include many genes that code for different…

Q: Use the illustration as a reference to select the correct statements transcription factors of…

A: Transcription is the process that is responsible for the production of RNA from a DNA template. It…

Q: 5' 3' ORF1 ORF2 ORF3 ORF4 ORF5 1. Above are pictured 2 operons, one that includes ORF1 and OFR2 and…

A: The production of RNA from DNA is known as transcription that occurs within the cytoplasm of…

Q: Let’s suppose a researcher was interested in the effects of mutationson the expression of a…

A: Western blot is a widely used biochemical technique to identify protein samples in cell extracts. It…

Q: You have isolated a protein that binds to DNA in the region upstream of the promoter sequence of a…

A: The promoter is the site of RNA polymerase binding that is very important step in the initiation…

Q: Strain LOLZ has a mutation in the lacI gene that results in a lacI protein that cannot bind…

A: Lac operon is the segment of DNA which is responsible for breaking down of lactose into glucose and…

Q: iRNAs are used to “knockdown” gene expression in research. Imagine you are a scientist who hopes to…

A: siRNA: si RNA stands for silencing or short interefing RNA is a double-stranded noncoding RNA that…

Q: During experimental RNAi, how does the researcher affect expression of a target gene? Group of…

A: Introduction: In the process of RNA interference(RNAi) double-stranded RNA molecule suppresses the…

Q: Why is there some signal present within the ‘no sugar’ and other noninducing sugars for the green…

A: The L-arabinose operon, commonly known as the ara operon, is a gene sequence that encodes enzymes…

Q: If telomeres are normally shortened after each round of replication in somatic cells (see Chapter…

A: Cloning is the creation of identical biological entities for therapeutic and reproduction. It is…

Q: Wild-type E. coli grow best at 37oC, but can grow efficiently at temperatures up to 42oC. An E. coli…

A: Mutations is the phenomenon in which small changes in the nucleotide sequences takes place which…

Q: An electrophoretic mobility shift assay can be used to study the bindingof proteins to a segment of…

A: RNA Polymerase II used in transcription process and produces mRNA, but this enzyme do not act alone,…

Q: Compare and contrast the use of histochemical reporter genes and fluorescent reporter genes — what…

A: Reporter genes can be analyzed by different methods, such as beta glucuronidase (uidA) gene…

Q: The eukaryotic chromosomes are linear DNA molecules with free 3'-OH and 5'-PO3- ends. DNA free ends…

A: According to our guidelines we are allowed to do one question or up to three sub apart of a…

Q: Remdesivir is a prodrug and not a substrate for the nsp12 polymerase. Why must remdesivir be…

A: Ramdesivir is a wide spectrum anti-viral nucleoside analogue drug which recently came into limelight…

Q: The 1000 aa protein (Protein X) you are studying has several putative caspase cleavage sites. You…

A: Western blotting is a molecular biology technique that is used to detect a specific protein from a…

Q: 20 ORF for gene X e for gene X above has a hypomorphic mutation (which you name Mutation #1) that is…

A: Answer.) :-The given question presents an experimental scenario involving Gene X, which has a…

Q: To identify the following types of genetic occurrences, would acomputer program use sequence…

A: Introduction  With the advancements of Bioinformatics tools and sequencing techniques we can easily…

Q: a eukaryotic protein involved in immunity, and you are attempting to express this protein in E. coli…

A: Escherichia coli is a prokaryotic organism (bacteria) which is a lab bacteria for carrying out…

Q: RNA polymerases

A: RNA polymerase:It is an important enzyme involved in transcription of DNA to RNA, gene expression.It…

Q: You design a guide RNA to target your gene of interest, which you will express attached to a…

A: CRISPR (clustered regularly interspaced short palindromic repeats)-Cas9 is a genome-editing tool. It…

Q: You have isolated a mutant that you call Mutant Z. In a Mutant Z strain, the RNA polymerase can bind…

A: Here, the correct answer is option c) domain 2 of sigma and d) 3/4 linker of sigma.

Q: propose a method with the help of which they can generate many copies of the gene.

A: The production of multiple copies of a gene from a single gene, this technique is called as DNA…

Q: 5' ATGATCTCGTAA 3' 3' TACTAGAGCATT 5'

A: DNA, or deoxyribonucleic acid, is a hereditary molecule that encodes the blueprint for the growth,…

Q: You are studying how the expression of Gene F is regulated. Initially, you identified three…

A: Step 1:To determine if any of the potential regulatory elements bind a transcriptional activator, we…

Q: The chart below is a position specific scoring matrix (PSSM, a logarithmic transformed matrix) for a…

A: Hi, Thanks For Your Question.  Q2. Answer : The Maximum Score That A Sequence Can Have With This…

Q: Primer 1 primes synthesis of the 5' to 3' strand (i.e. sense strand) of an oat cDNA/gene and encodes…

A: A primer is sometimes known to state because the short sequence of nucleic acids that's the…

Q: Knock out mice that are mutant for a gene product X die. Variants of the X gene that were…

A: The physical crossing over during meiosis of parental heterozygous homologous chromosomes or genes…

Q: was reduced in the knock-out mutant cells. The N-terminal region of Protein X is 100% conserved…

A: As per our policy we are answering the first three sub parts ( a, b , c ). Of the Question For the…

Q: True/False: In general, weak promoters that support active transcription have -10 and -35 boxes that…

A: Promoters are DNA sequences that are located upstream of the transcription start site of a gene and…

Q: A tumour inducing plasmid shown in Figure 2 is isolated from a soil bacterium. (i) Auxin Left Border…

A: A plasmid vector must have a single replication origin, a cloning site (where restriction enzymes…

Q: 1.Your goal is to design a strategy to create thisfinal pDHFR plasmid for fusion protein…

A: Vectors are DNA molecules that act as transporting vehicle which carries foreign DNA into a host…

Q: In mammalian cells, genes that are expressed in a particular cell are reported to undergo…

A: Mammalian cells : It are cells that are derived or isolated from tissue of a mammal. In this…

Q: Given the Table below concerning the lac operon, match the letter with what would be occurring for…

A: The lac operon is a genetic system found in bacteria, such as E. coli, that regulates the expression…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.