According to the scenario shown, how many segments of DNA (one, two, or three) are removed during site-specific recombination within the gene that encodes the κ (kappa) light chain for IgG proteins? How many segments are spliced out of the pre-mRNA? Organization of domains in the precursor gene for the x light chainV3 Va V300J2 Ja JAcRAG1 and RAG2 recognize recombinationsignal sequences and catalyze thebreakage at the end of a variable domainand beginning of a joining domain. In thisexample, it occurs at the end of V7g andbeginning of J2.V V2V3 Va V78The intervening DNA is lost. NHEJproteins catalyze the joining of thelast V domain and first J domain inV300the remaining DNA.The gene is transcribed into apre-MRNA starting at the lastvariable domain.V78 J2J33'5'The region between the first joiningdomain and constant domain in thepre-MRNA is spliced out.3'V78 J2The mRNA is translated into apolypeptide containing 1 variable,1 joining, and 1 constant domain. NH,V78Vтв Ja-соон2 light polypeptides and 2heavy polypeptides assemble toform a functional antibody protein.NH2NH2HeavypolypeptideСООН-COOH A functionaantibody-COOH made in1B cellСООнNH,-`LightpolypeptideNHFIGURE 20.7 Site-specific recombination within the precur-sor gene that encodes the x light chain for immunoglobulin G (IgG)in the mouse.

Name: According to the scenario shown, how many segments of DNA (one, two, o
Uploaded: 2024-03-20T06:42:41.000Z
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Description: According to the scenario shown, how many segments of DNA (one, two, or three) are removed during site-specific recombination within the gene that encodes the κ (kappa) light chain for IgG proteins? How many segments are spliced out of the pre-mRNA?