Another mutant for of the Lac promoter demonstrates the characteristics below compared to the wild-type promoter. The RNA Polymerase Holoenzyme binds tighter to the mutant promoter, but has reduced promoter activity in an assay with the promoter fuse to the gene for Green Fluorescent Protein (GFP). Provide a plausible explanation for this data. Lac Promoter Activity punog Binding of RNA Pol Holoenzymes to Promoters 120 100 80 60 40 Lac Promoter X GFP Fluorecence 100 90 80 70 60 50 40 30

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Another mutant for of the Lac promoter demonstrates the characteristics below compared to the wild-type promoter. The RNA
Polymerase Holoenzyme binds tighter to the mutant promoter, but has reduced promoter activity in an assay with the promoter fuse
to the gene for Green Fluorescent Protein (GFP). Provide a plausible explanation for this data.
Lac Promoter Activity
punog
Binding of RNA Pol Holoenzymes to
Promoters
120
100
80
60
40
20
0
0
20
40
Lac
Promoter X
60
[DNA] nM
80
100
120
GFP Fluorecence
100
90
80
70
60
50
40
30
20
10
0
Lac
Promoter X
Transcribed Image Text:Another mutant for of the Lac promoter demonstrates the characteristics below compared to the wild-type promoter. The RNA Polymerase Holoenzyme binds tighter to the mutant promoter, but has reduced promoter activity in an assay with the promoter fuse to the gene for Green Fluorescent Protein (GFP). Provide a plausible explanation for this data. Lac Promoter Activity punog Binding of RNA Pol Holoenzymes to Promoters 120 100 80 60 40 20 0 0 20 40 Lac Promoter X 60 [DNA] nM 80 100 120 GFP Fluorecence 100 90 80 70 60 50 40 30 20 10 0 Lac Promoter X
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