. One way to determine where inside a cell a protein(protein X) normally localizes is to generate a reportergene construct containing: (i) the gene X regulatoryregion and coding sequences, and (ii) coding sequencesfor GFP fused in frame to the 3′ end of the gene Xcoding sequences just before the stop codon. A mousecontaining such a transgene will express a hybrid protein X-GFP only in those cells in which gene X is normally expressed.a. The gene X-GFP fusion gene described could begenerated by knocking in GFP coding sequencesinstead of by random insertion of a transgene.Diagram the knockin construct you could use forthis purpose.b. What might the advantage be of the knockin strategy versus the transgene strategy?
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
. One way to determine where inside a cell a protein
(protein X) normally localizes is to generate a reporter
gene construct containing: (i) the gene X regulatory
region and coding sequences, and (ii) coding sequences
for GFP fused in frame to the 3′ end of the gene X
coding sequences just before the stop codon. A mouse
containing such a transgene will express a hybrid protein X-GFP only in those cells in which gene X is normally expressed.
a. The gene X-GFP fusion gene described could be
generated by knocking in GFP coding sequences
instead of by random insertion of a transgene.
Diagram the knockin construct you could use for
this purpose.
b. What might the advantage be of the knockin strategy versus the transgene strategy?
Trending now
This is a popular solution!
Step by step
Solved in 2 steps with 1 images
