One can synthesize RNA from any gene in a test tube (in vitro). You want to synthesize mRNA from a prokaryotic gene in a test tube. You added the gene, which contains its promoter, coding region, and rho-dependent transcription termination site, in a test tube. Which of the following do you need to add to this test tube so that you can synthesize RNA from this gene and terminate transcription accurately? 1) RNA polymerase core enzyme, 2) RNA polymerase holoenzyme,3) RNA polymerase II, 4) ATP, CTP, GTP, and TTP mixture 5) ATP, GTP, CTP, and UTP mixture, 6) dATP, dGTP, dCTP, and dUTP mixture 7) Rho factor, 8) primer. 3,4,7 1,6,7 O2,5,7 2.4.6 2,5,7,8

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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To synthesize RNA from any gene in a test tube (*in vitro*), you want to synthesize mRNA from a prokaryotic gene in a test tube. You added the gene, which contains its promoter, coding region, and rho-dependent transcription termination site, in a test tube. Which of the following do you need to add to this test tube so that you can synthesize RNA from this gene and terminate transcription accurately?

1) RNA polymerase core enzyme
2) RNA polymerase holoenzyme
3) RNA polymerase II
4) ATP, CTP, GTP, and TTP mixture
5) ATP, GTP, CTP, and UTP mixture
6) dATP, dGTP, dCTP, and dUTP mixture
7) Rho factor
8) Primer

**Options:**

- ○ 3, 4, 7
- ○ 1, 6, 7
- ● 2, 5, 7
- ○ 2, 4, 6
- ○ 2, 5, 7, 8

The selected option is: 2, 5, 7.
Transcribed Image Text:**Transcription of Image for Educational Website:** To synthesize RNA from any gene in a test tube (*in vitro*), you want to synthesize mRNA from a prokaryotic gene in a test tube. You added the gene, which contains its promoter, coding region, and rho-dependent transcription termination site, in a test tube. Which of the following do you need to add to this test tube so that you can synthesize RNA from this gene and terminate transcription accurately? 1) RNA polymerase core enzyme 2) RNA polymerase holoenzyme 3) RNA polymerase II 4) ATP, CTP, GTP, and TTP mixture 5) ATP, GTP, CTP, and UTP mixture 6) dATP, dGTP, dCTP, and dUTP mixture 7) Rho factor 8) Primer **Options:** - ○ 3, 4, 7 - ○ 1, 6, 7 - ● 2, 5, 7 - ○ 2, 4, 6 - ○ 2, 5, 7, 8 The selected option is: 2, 5, 7.
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