Now you have the gene sequence. Now you would like to clone it into an expression vector to grow up in a bacterial system. Because you're going to use bacteria to generate protein from a eukaryote, the mammoth, you need to get rid of introns from your sequence. How do you do that? Bioinformatically, I look for splice-site sequences and branch-point adenines and predict intron-exon boundaries I use a comparative genomic approach and use sequence homology with the genome of a closely related species I use a comparative genomic approach and use sequence homology with the genome of a distantly related species Both A and B Both B and C Why did you bother to identify the introns? So that I could include them in the sequence to understand intron function. So that I could exclude them from the sequence because prokaryotes don't have spliceosomal machinery. So that I could see how introns affect protein folding.

Answer. The majority of eukaryotic genes contain non-coding DNA. The genes tend to have a split…

Answered: Now you have the gene sequence. Now you…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Now you have the gene sequence. Now you would like to clone it into an expression vector to grow up in a bacterial system. Because you're going to use bacteria to generate protein from a eukaryote, the mammoth, you need to get rid of introns from your sequence. How do you do that?

		Bioinformatically, I look for splice-site sequences and branch-point adenines and predict intron-exon boundaries
		I use a comparative genomic approach and use sequence homology with the genome of a closely related species
		I use a comparative genomic approach and use sequence homology with the genome of a distantly related species
		Both A and B
		Both B and C

Why did you bother to identify the introns?

		So that I could include them in the sequence to understand intron function.
		So that I could exclude them from the sequence because prokaryotes don't have spliceosomal machinery.
		So that I could see how introns affect protein folding.

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