Your research project involves adding a salamander gene to a bacterial cell so that it gets translated into a protein for biochemical analyses. Point out three things that you will need to change in the sequence of this typical eukaryotic gene in order for it to be correctly transcribed and translated in a bacterium. Place gene inside expression vector, then insert CDNA into expression vector, and finally encode with ribosome binding site near initiation codon.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Your research project involves adding a salamander gene to a bacterial cell so that it gets translated into a
protein for biochemical analyses. Point out three things that you will need to change in the sequence of this
typical eukaryotic gene in order for it to be correctly transcribed and translated in a bacterium.
Place gene inside expression vector, then insert CDNA into expression vector, and finally encode with
ribosome binding site near initiation codon.
Transcribed Image Text:Your research project involves adding a salamander gene to a bacterial cell so that it gets translated into a protein for biochemical analyses. Point out three things that you will need to change in the sequence of this typical eukaryotic gene in order for it to be correctly transcribed and translated in a bacterium. Place gene inside expression vector, then insert CDNA into expression vector, and finally encode with ribosome binding site near initiation codon.
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