You get the sequence of a gene with 10 exons, 9 introns, and a polyadenylation consensus sequence in the 3 UTR. A polyadenylation site is also found in the fifth intron. You separate mRNA and discover a longer transcript from muscle tissue and a shorter mRNA transcript from all other tissues to see whether both polyadenylation sites are utilised. Consider the process that results in the generation of these two transcripts.

Q: One can synthesize RNA from any gene in a test tube (in vitro). You want to synthesize mRNA from a…

A: Translation is a heterocatalytic action of DNA by means of which RNA is synthesize from specific DNA…

Q: Describe the difference between a transcriptional fusion and a translational reporter gene fusion.…

A: Transcription fusion means cloning the promoter of interest, upstream of any transcription unit,…

Q: O a base substitution in an intron 5' splice site O a frameshift in an exon O a missense in an exon…

A: The answer to the question is  A frameshift in exon

Q: Consider the Rho-dependent terminator sequence 5’CCCAGCCCGCCUAAUGAGCGGCCUUUUUUUU-3’. What affect…

A: The mutation is the change in the nucleotide sequence of the DNA. The point mutation is the mutation…

Q: ALTE

A: Exon is a genetic code region that contains the information needed to enter a protein code. In…

Q: Examine the following mRNA transcripts: Wild type: 5' CACUGAUGCACGGAUCAU 3' Mutant: 5'…

A:

Q: Suppose that the diagram below represents the genomic organization of an enzyme involved in eye…

A: Transcription is the process by which  RNA transcript is formed from DNA. Various type of RNA are…

Q: A eukaryotic gene has two introns and three exons. The first intron closest to the promoter is 157…

A: To draw the structure of the hybridized mRNA and estimate the size of the protein coded for by this…

Q: . The human gene for ß2 lens crystallin has the components listed below. The numbers represent…

A: Since we only answer up to 3 sub-parts, we’ll answer the first three. Please resubmit the question…

Q: Given the mRNA sequence: 5' UAGUUUCAAGU 3', which of the answers below represents the corresponding…

A: The first stage of DNA-based gene expression is transcription, in which a portion (or the whole…

Q: A rice MADS box transcription factor gene contains a number of sequences that are removed in the…

A: In eukaryotes including rice mRNA processing involves the removal of introns which are non-coding…

Q: You have cloned a novel E. coli gene and wish to express protein from it in human cells. In order to…

A: The Central Dogma theory explains that DNA forms DNA by Replication, DNA forms RNA by Transcription…

Q: Once transcribed, the length of the mRNA for gene X is usually 1000 nucleotides long in your…

A: Rho utilization site , also known by the acronym rut is a sequence of RNA in bacteria upstream of…

Q: protein. How would the protein produced from sequence #1 differ from the protein produced from…

A: The sequences provided are of a non-coding strand or template strand. mRNA is transcribed…

Q: You want to examine genetic variation in your gene promoter. You sequence DNA from several…

A: Single nucleotide polymorphism; it is a type of genetic variation which can occur throughout a…

Q: a gene sequence has two poly-A sites, one in the center and one at the end, we can isolate mRNA with…

A: Poly-A refers to the poly-A tail which is at the 3'-end of oligonucleotides consisting of a…

Q: ncer where the newly-discovered genes BR and AIN are known to play a role in the development of this…

A: Cancer results from the uncontrolled cell division giving rise to a lump of cells that may be…

Q: Now you have the gene sequence. Now you would like to clone it into an expression vector to grow up…

A: Answer. The majority of eukaryotic genes contain non-coding DNA. The genes tend to have a split…

Q: What is the length (in amino acids) of the protein* encoded by the mature processed mRNA? *Assume…

A: To calculate the length of the protein encoded by the mature processed mRNA, we need to consider the…

Q: Suppose that a consensus sequence in the regulatory promotor of a eukaryotic gene that encodes an…

A: Gene transcription i.e., mRNA formation from the DNA required many transcription factors and RNA…

Q: The mRNA sequence 5' AUG AAA CAG GGA UAA 3' encodes a particular peptide of interest to your…

A: Gene is the segment of DNA (deoxyribonucleic acid) that is responsible for heredity and inheritance…