Many regions of non-coding eukaryotic DNA previously thought to be "junk" are now known to contain sequence elements important to regulating gene expression. What approach can be used to identify important non-coding regulatory regions when annotating a newly sequenced genome? O comparing CDNA to genomic DNA to validate that the gene is expressed O identifying restriction enzyme recognition sequences in the genome O phylogenetic footprinting to identify conserved non-coding sequences O searching for start/stop codons and splice recognition sites that predict where a gene might be located

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