Using RNA interference, it is possible to significantly reduce homologous mRNA transcripts and thereby reduce the expression level of a specific gene of interest. What methods could be used to confirm that an RNAi knockdown experiment has been successful? genome sequencing labeling the RNAi construct western blotting quantitative real-time PCR The relative ease of RNAi studies means that large-scale screens can be performed to assess a wide array of targets. What source would produce the most functional RNAi library for conducting genome-wide screens? microRNAs whole-genome proteins whole-genome DNA whole-genome cDNA

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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### RNA Interference and Gene Expression

**Introduction to RNA Interference (RNAi):**
Using RNA interference, it is possible to significantly reduce homologous mRNA transcripts and thereby reduce the expression level of a specific gene of interest.

**Methods to Confirm Successful RNAi Knockdown:**
- What methods could be used to confirm that an RNAi knockdown experiment has been successful?
  - [ ] Genome sequencing
  - [ ] Labeling the RNAi construct
  - [ ] Western blotting
  - [ ] Quantitative real-time PCR

**Advantages of RNAi Studies:**
The relative ease of RNAi studies means that large-scale screens can be performed to assess a wide array of targets.

**Sources for Functional RNAi Library:**
- What source would produce the most functional RNAi library for conducting genome-wide screens?
  - ( ) microRNAs
  - ( ) Whole-genome proteins
  - ( ) Whole-genome DNA
  - ( ) Whole-genome cDNA

This information highlights the utility of RNA interference in gene research and the various techniques available to validate and utilize RNAi experiments effectively.
Transcribed Image Text:### RNA Interference and Gene Expression **Introduction to RNA Interference (RNAi):** Using RNA interference, it is possible to significantly reduce homologous mRNA transcripts and thereby reduce the expression level of a specific gene of interest. **Methods to Confirm Successful RNAi Knockdown:** - What methods could be used to confirm that an RNAi knockdown experiment has been successful? - [ ] Genome sequencing - [ ] Labeling the RNAi construct - [ ] Western blotting - [ ] Quantitative real-time PCR **Advantages of RNAi Studies:** The relative ease of RNAi studies means that large-scale screens can be performed to assess a wide array of targets. **Sources for Functional RNAi Library:** - What source would produce the most functional RNAi library for conducting genome-wide screens? - ( ) microRNAs - ( ) Whole-genome proteins - ( ) Whole-genome DNA - ( ) Whole-genome cDNA This information highlights the utility of RNA interference in gene research and the various techniques available to validate and utilize RNAi experiments effectively.
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