Using RNA interference, it is possible to significantly reduce homologous mRNA transcripts and thereby reduce the expression level of a specific gene of interest. What methods could be used to confirm that an RNAi knockdown experiment has been successful? genome sequencing labeling the RNAi construct western blotting quantitative real-time PCR The relative ease of RNAi studies means that large-scale screens can be performed to assess a wide array of targets. What source would produce the most functional RNAi library for conducting genome-wide screens? microRNAs whole-genome proteins whole-genome DNA whole-genome cDNA
Using RNA interference, it is possible to significantly reduce homologous mRNA transcripts and thereby reduce the expression level of a specific gene of interest. What methods could be used to confirm that an RNAi knockdown experiment has been successful? genome sequencing labeling the RNAi construct western blotting quantitative real-time PCR The relative ease of RNAi studies means that large-scale screens can be performed to assess a wide array of targets. What source would produce the most functional RNAi library for conducting genome-wide screens? microRNAs whole-genome proteins whole-genome DNA whole-genome cDNA
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![### RNA Interference and Gene Expression
**Introduction to RNA Interference (RNAi):**
Using RNA interference, it is possible to significantly reduce homologous mRNA transcripts and thereby reduce the expression level of a specific gene of interest.
**Methods to Confirm Successful RNAi Knockdown:**
- What methods could be used to confirm that an RNAi knockdown experiment has been successful?
- [ ] Genome sequencing
- [ ] Labeling the RNAi construct
- [ ] Western blotting
- [ ] Quantitative real-time PCR
**Advantages of RNAi Studies:**
The relative ease of RNAi studies means that large-scale screens can be performed to assess a wide array of targets.
**Sources for Functional RNAi Library:**
- What source would produce the most functional RNAi library for conducting genome-wide screens?
- ( ) microRNAs
- ( ) Whole-genome proteins
- ( ) Whole-genome DNA
- ( ) Whole-genome cDNA
This information highlights the utility of RNA interference in gene research and the various techniques available to validate and utilize RNAi experiments effectively.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F049a8034-a0ab-4b8d-b3d1-3977f5f9e464%2F968f425a-181e-4829-a221-7dd6b4f7e1f0%2F2qye4mo_processed.png&w=3840&q=75)
Transcribed Image Text:### RNA Interference and Gene Expression
**Introduction to RNA Interference (RNAi):**
Using RNA interference, it is possible to significantly reduce homologous mRNA transcripts and thereby reduce the expression level of a specific gene of interest.
**Methods to Confirm Successful RNAi Knockdown:**
- What methods could be used to confirm that an RNAi knockdown experiment has been successful?
- [ ] Genome sequencing
- [ ] Labeling the RNAi construct
- [ ] Western blotting
- [ ] Quantitative real-time PCR
**Advantages of RNAi Studies:**
The relative ease of RNAi studies means that large-scale screens can be performed to assess a wide array of targets.
**Sources for Functional RNAi Library:**
- What source would produce the most functional RNAi library for conducting genome-wide screens?
- ( ) microRNAs
- ( ) Whole-genome proteins
- ( ) Whole-genome DNA
- ( ) Whole-genome cDNA
This information highlights the utility of RNA interference in gene research and the various techniques available to validate and utilize RNAi experiments effectively.
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