RNA sequencing can detect DNA mutations typically easier to analyze can detect transcripts with very low abundance Both Answer Bank used for gene-expression analysis Microarray requires transcript-specific probes

Q: What would three possible reasons be that a PCR wouldn't work after cloning a TAQ sequence into a…

A: Even though you've verified Taq protein expression through SDS-PAGE, your PCR might still fail for a…

Q: Mediator is not necessary to reproduce transcription initiation reactions in vitro. If you are…

A: In the study of eukaryotic gene expression, understanding the process of transcription initiation is…

Q: Mapping high-throughput sequencing reads (choose all that apply) O requires a special device called…

A: Mapping high throughput sequencing refers to the amount of DNA molecules that read at the same time.…

Q: Define these terms and if it is a technique in genomics explain what it is used for. siRNA paralog…

A: 1) siRNA = known as short interfering RNA or silencing RNA or small interfering RNA. Function : it…

Q: Describe the banding pattern seen in the gel. What does that tell you about the role of annealing…

A: Gel electrophoresis is a laboratory technique for separating charged molecules such as DNA, RNA, and…

Q: You complete a comparative transcriptomics experiment, where RNA from a plant sample grown in sandy…

A: The researchers compared the gene expression differences between plants cultivated in silty soil and…

Q: True or False: 1. The 6X HIS tag is required for a eukaryotic protein to be expressed in E. coli 2.…

A: Polyhistidine tag is added to a protein and helps in its purification. A recombinant protein can…

Q: Even though RNA degrades quickly (unstable) and is tedious to isolate compared to DNA isolation, why…

A: As structure of RNA is similar to DNA only difference that RNA is single stranded and DNA is double…

Q: Describe what CRISPR is and explain the overall process of how it works. Identify and discuss 3-4…

A: CRISPR : It stands for Clustered Regularly Interspaced Short Palindromic Repeats  Repetitive DNA…

Q: 23. Describe each type of transposon and how they integrate into the DNA. How are transposons…

A: Genome is defined as the complete set of genetic information that make up all the chromosomes of the…

Q: Real-time PCR is used in many transcription? Select one or more: O a. Gene transcription qu b. Ob.…

A: Note- According to the guidelines only one question can be answered at a time. Hence, the first…

Q: please help solve

A: CRISPR-Cas system is the naturally present defense system in the prokaryotes.

Q: Restriction digestion of DNA fragments is not sequence specific. True False

A: Within a few years after discovering EcoB, EcoK, and HindII, scientists were already experimenting…

Q: What are the different types of fermentation pathways and how can they be used to differentiate…

A: Fermentation is the process of conversion of complex molecules into simpler form under anaerobic…

Q: LO65 Compare methods that Covid tests and Covid vaccines use.

A: Comparison of methods that Covid tests and Covid vaccines use. there are several test  method used…

Q: Which of the following is a correct statement about CRISPR-Cas-9 gene editing? Group of answer…

A: CRISPR-Cas9 is a widely used genome editing technology as it enables scientists to alter genes in…

Q: How many restrictions sites are present in the EcoRI. Calculate the number of restriction sites for…

A: Restriction endonucleases or restriction enzymes are enzymes that cleave or cut nucleic acids at…

Q: Using the diagram below describe the significance of DNA sequencing for identification of mutations.

A: DNA sequencing is typically the process of determining the nucleic acid sequence the order of…

Q: Match the following (most appropriate combinations): Gel filteration chromatography Electrophoresis…

A: 1) Gel filtration chromatography - separation of proteins in a column based on size. In this type of…

Q: 1)explain why it is far less important for RNA Polymerase to have proofreading activity than it is…

A: Fidelity of replication is the most important for the very existence of an organism. Besides its…

Q: It is true in RNAsrq technology ans microsrray..... both assess gene expression in protein…

A: Gene expression is the phenomena by which a gene gets activated in a cell to make RNA and proteins.…

Q: Provide the SIGNIFICANT difference between the terms provided in the table below. Missense Nonsense…

A: Missense :  Missense mutation changes an amino acid to another amino acid.     Nonsense : Nonsense…

Q: fragmentation

A: For solving this question one must be aware of the working of the RNAi where 'i' stand for…

Q: What gene sequences are selected during DNA barcoding?

A: DNA barcoding is a taxonomic technique which identifies at the species level by using a brief…

Q: What is the role of the following molecules in the CRISPR mechanism? *

A: CRISPR stands for clustered regularly interspaced short palindromic repeats. These are a set of DNA…

Q: During the differential sene expression module.which of the following was used as template in the…

A: Differential gene expression is the process through which cells differ from one another because of…

Q: pls help very urgent, no explaantion required, pls help asap

A: In molecular biology, a restriction digest is a technique for preparing DNA for analysis or further…

Q: Match each description with the most appropriate term. Cuts apart foreign DNA as its primary…

A: There are several key terms and concepts in molecular biology that must be understood. This includes…

Q: A mulitple cloning site (MSC) is used in TOPA-TA cloning in TA cloning with restriction enzymes both…

A: The process by which genetic material from one organism is introduced into another organism to…

Q: Pls help ASAP

A: Restrictions enzymes are bacterial proteins that cleave or fragment DNA at particular locations.…

Q: ZFNs What is it? And how it works in genome editing?

A: Zinc-finger nucleases are counterfeit limitation catalysts (artificial restriction enzyme) produced…

Q: Enhancer sequences may be very far from the genes they affect but they are always upstream of the…

A: Enhancer sequence : These are regulatory DNA sequences, when these are bound by transcription…

Q: The PCR process has 4 steps :collection, preparation, amplification, and post PCR clean-up. The PCR…

A: PCR stands for Polymerase Chain Reaction. PCR is a technique used to rapidly amplify specific…

Q: Which of the following is not true about microarrays? Group of answer choices A microarray is a…

A: Introduction :- DNA microarray ( also known as gene chip , DNA chip, bioarray or gene array ) is an…

Q: Which of the following tools should be used in identifying a mutation in a sequence compared to a…

A: Gene mutations, and alterations in the DNA sequence, play a fundamental role in shaping life and…

Q: Select the correct statements about CRISPR-Cas9 CRISPR-Cas9 can only introduce mutations in the…

A: CRISPR-Cas9 is a gene-editing tool that scientists use to make precise changes to DNA. It uses a…

Q: CRISPR-Cas9 technology can edit the DNA in random regions  True  False

A: CRISPR Cas9 technology can edit DNA in precise specific locations and not rand DNA regions. CRISPR…

Q: Part A Why are most recombinant human proteins produced in animal or plant hosts instead of…

A: Bacteria is a prokaryote and human cells are eukaryotic. There is a significant difference in the…

Q: STR markers: are point mutations detectable by DNA sequencing are variations in the number of…

A: STR markers, also known as short tandem repeat markers, are regions of DNA that contain a pattern of…

Q: To study the function of any gene of interest you would perform the loss and gain of function…

A: Genes : It can be defined as a segment of DNA that codes for a specific protein performing a…

Q: Transcribe and translate the mutated sequence #2. Determine the consequence, if any, for each…

A: DNA or Deoxyribonucleic acid serves as the genetic material of biological entities. It is composed…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.