Explain the steps of RNA-Sequencing down below, and explain the steps of anylazing the data. ### mRNA Sequencing WorkflowThis diagram illustrates the process of mRNA sequencing from cultured cells.1. **Cultured Cells to Cell Lysate**: - Begin with cultured cells. - Cells are lysed to produce a cell lysate for mRNA isolation.2. **mRNA Isolation**: - mRNA is isolated from the cell lysate.3. **Fragment mRNA**: - The isolated mRNA is fragmented into smaller pieces.4. **Convert to cDNA and Add Linkers**: - Fragmented mRNA is reverse transcribed into complementary DNA (cDNA). - Linkers are added to the cDNA fragments.5. **PCR Amplification**: - The cDNA is amplified using PCR with linker primers to increase the amount of DNA available for sequencing.6. **High-Throughput cDNA Sequencing**: - The amplified cDNA is sequenced using high-throughput sequencing technologies, producing short cDNA sequences.7. **Map cDNA Sequences onto Genome**: - The short cDNA sequences are mapped back to the genomic sequence.8. **Quantify mRNA Transcripts**: - The number of mRNA transcripts in the sample is quantified by examining how many times each cDNA sequence aligns with regions of the genome, such as exons.This process allows researchers to analyze the expression levels of genes within a cell or tissue by measuring the abundance of mRNA transcripts.

Answered: Convert to cDNA and add linkers

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Related questions

Concept explainers

Question

Explain the steps of RNA-Sequencing down below, and explain the steps of anylazing the data.

### mRNA Sequencing Workflow

This diagram illustrates the process of mRNA sequencing from cultured cells.

1. **Cultured Cells to Cell Lysate**:
- Begin with cultured cells.
- Cells are lysed to produce a cell lysate for mRNA isolation.

2. **mRNA Isolation**:
- mRNA is isolated from the cell lysate.

3. **Fragment mRNA**:
- The isolated mRNA is fragmented into smaller pieces.

4. **Convert to cDNA and Add Linkers**:
- Fragmented mRNA is reverse transcribed into complementary DNA (cDNA).
- Linkers are added to the cDNA fragments.

5. **PCR Amplification**:
- The cDNA is amplified using PCR with linker primers to increase the amount of DNA available for sequencing.

6. **High-Throughput cDNA Sequencing**:
- The amplified cDNA is sequenced using high-throughput sequencing technologies, producing short cDNA sequences.

7. **Map cDNA Sequences onto Genome**:
- The short cDNA sequences are mapped back to the genomic sequence.

8. **Quantify mRNA Transcripts**:
- The number of mRNA transcripts in the sample is quantified by examining how many times each cDNA sequence aligns with regions of the genome, such as exons.

This process allows researchers to analyze the expression levels of genes within a cell or tissue by measuring the abundance of mRNA transcripts.

Expert Solution

Step 1

This is the High-throughput sequencing that analyzes DNA/RNA genome and becomes the standard tool for gene analyses in the biomedical fields. Since it is a single sequencing run that is sufficient for analyzing the multiple samples, the individual samples are cloned with specific barcodes pooled and sequenced as a single library at low cost, and then debarcoded obtained the sample-specific sequences.

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions