Transcribe and translate the mutated sequence #2. Determine the consequence, if any, for each mutation. You will need to use your codon charts in you ISN. Practice with both charts. Original DNA sequence: mRNA transcript: amino acids: Mutated DNA sequence #2: mRNA transcript: (Circle any changes) amino acids: Type of mutation (Circle one.) How did the mutation affect the amino acid sequence (protein)? (Circle all that apply.) ARG Point TAC AUG METH TAC Silent (No change) ACC UGG TRP GAC Substitution Missense (1 amino acid changed) TTG AAC CUG :: PRO :: AUG :: ASN ASN CTT Nonsense (Premature stop) signal GCG CGC ARG GGC Frameshift No stop signal ACG UGC CYS GAC ACT CCG :: GAA :: GLU UGA STOP GAC T Insertion or 1 amino acid added/ deleted LEU Deletion All the amino acids changed after the point of mutation :: Stop ::Start
Transcribe and translate the mutated sequence # 2. Determine the consequence, if any, for each mutation. You wi ISN. Practice with both charts. Original DNA sequence: mRNA transcript: amino acids: Mutated DNA sequence #2: mRNA transcript: (Circle any changes) amino acids: Type of mutation (Circle one.) How did the mutation affect the amino acid sequence (protein)? (Circle all that apply.) #ARG Point TAC AUG METH TAC Silent (No change) || || CUG #PRO ACC UGG TRP GAC Substitution Missense (1 amino acid changed) #AUG TTG AAC ASN ASN CTT Nonsense (Premature stop) signal GCG CGC ARG GGC Frameshift No stop signal ACG UGC CYS GAC GAC # CCG #GAA #GLU Inserti 1 amino ac added/ deleted LE
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