You would like to design a method to treat the most common mutation that causes sickle cell anemia using CRISPR/Cas. This mutation is a missense mutation that changes a glutamic acid to a valine in the beta-globin coding sequence, resulting in a non functional protein product. You remove blood stem cells from a patient and use a virus to insert which of the following sequences into the stem cells (select all sequences you need). Guide RNA that matches the mutated gene Donor DNA with mutant DNA Cas9 gene Donor DNA with wild type DNA sequence RNAi for the mutated gene
You would like to design a method to treat the most common mutation that causes sickle cell anemia using CRISPR/Cas. This mutation is a missense mutation that changes a glutamic acid to a valine in the beta-globin coding sequence, resulting in a non functional protein product. You remove blood stem cells from a patient and use a virus to insert which of the following sequences into the stem cells (select all sequences you need). Guide RNA that matches the mutated gene Donor DNA with mutant DNA Cas9 gene Donor DNA with wild type DNA sequence RNAi for the mutated gene
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter13: An Introduction To Genetic Technology
Section: Chapter Questions
Problem 20QP: Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant...
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![You would like to design a method to treat the most common mutation that
causes sickle cell anemia using CRISPR/Cas. This mutation is a missense
mutation that changes a glutamic acid to a valine in the beta-globin coding
sequence, resulting in a non functional protein product. You remove blood
stem cells from a patient and use a virus to insert which of the following
sequences into the stem cells (select all sequences you need).
Guide RNA that matches the mutated gene
Donor DNA with mutant DNA
Cas9 gene
Donor DNA with wild type DNA sequence
RNAi for the mutated gene](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Faefc02fc-f1ae-4f90-a486-edd86b942252%2Fe63d5abe-15e0-4df9-9637-78da15e5b300%2F0aozti_processed.jpeg&w=3840&q=75)
Transcribed Image Text:You would like to design a method to treat the most common mutation that
causes sickle cell anemia using CRISPR/Cas. This mutation is a missense
mutation that changes a glutamic acid to a valine in the beta-globin coding
sequence, resulting in a non functional protein product. You remove blood
stem cells from a patient and use a virus to insert which of the following
sequences into the stem cells (select all sequences you need).
Guide RNA that matches the mutated gene
Donor DNA with mutant DNA
Cas9 gene
Donor DNA with wild type DNA sequence
RNAi for the mutated gene
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