You would like to design a method to treat the most common mutation that causes sickle cell anemia using CRISPR/Cas. This mutation is a missense mutation that changes a glutamic acid to a valine in the beta-globin coding sequence, resulting in a non functional protein product. You remove blood stem cells from a patient and use a virus to insert which of the following sequences into the stem cells (select all sequences you need). Guide RNA that matches the mutated gene Donor DNA with mutant DNA Cas9 gene Donor DNA with wild type DNA sequence RNAi for the mutated gene

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Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.