Homework Help is Here – Start Your Trial Now!
Science
Biology
1. Create an inversion on your mini gene ... ? that results in an inversion. Sequence 5 ATGGTGCAAGATTAA 3' 3 TACCACGTTCTAATT 5' The sequence after the inversion is: 5 ATGCTTACGGATTAA 3' 3' TACGAATGCCTAATT 5' 2. Generate a primer pair that is 6 base-pairs long to amplify the inversion you created, that will no amplify your wildtype mini gene. 3. Create point mutations on the sequence on you minigene that results in: i. a frameshift, ii. a silent mutation, iii. a conservative mutation, iv. missense, v. a nonsense mutation.

1. Create an inversion on your mini gene ... ? that results in an inversion. Sequence 5 ATGGTGCAAGATTAA 3' 3 TACCACGTTCTAATT 5' The sequence after the inversion is: 5 ATGCTTACGGATTAA 3' 3' TACGAATGCCTAATT 5' 2. Generate a primer pair that is 6 base-pairs long to amplify the inversion you created, that will no amplify your wildtype mini gene. 3. Create point mutations on the sequence on you minigene that results in: i. a frameshift, ii. a silent mutation, iii. a conservative mutation, iv. missense, v. a nonsense mutation.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question
1. Create an inversion on your mini gene .. ? that results in an inversion. Sequence 5 ATGGTGCAAGATTAA 3' 3 TACCACGTTCTAATT 5' The sequence after the inversion is: 5 ATGCTTACGGATTAA 3 3 TACGAATGCCTAATT 5' Comments and help • By convention we write DNA sequences starting with the 5' end and ending at the 3' end. 4. Create mutation on the sequence on your minigene that results in an in-frame non-tandem duplication. • Also, by convention when you write double stranded sequences, the top strand is the 5' to 3' and the bottom strand 3' to 5'. 2. Generate a primer pair that is 6 base-pairs long to amplify the inversion you created, that will not amplify your wildtype mini gene. • If you are typing your answer, use the Courier font for the sequence. This allows proper alignment of the two strands. 3. Create point mutations on the sequence on your > minigene that results in: i. a frameshift, ii. a silent mutation, iii. a conservative mutation, iv. a missense, v. a nonsense mutation.
Transcribed Image Text:1. Create an inversion on your mini gene .. ? that results in an inversion. Sequence 5 ATGGTGCAAGATTAA 3' 3 TACCACGTTCTAATT 5' The sequence after the inversion is: 5 ATGCTTACGGATTAA 3 3 TACGAATGCCTAATT 5' Comments and help • By convention we write DNA sequences starting with the 5' end and ending at the 3' end. 4. Create mutation on the sequence on your minigene that results in an in-frame non-tandem duplication. • Also, by convention when you write double stranded sequences, the top strand is the 5' to 3' and the bottom strand 3' to 5'. 2. Generate a primer pair that is 6 base-pairs long to amplify the inversion you created, that will not amplify your wildtype mini gene. • If you are typing your answer, use the Courier font for the sequence. This allows proper alignment of the two strands. 3. Create point mutations on the sequence on your > minigene that results in: i. a frameshift, ii. a silent mutation, iii. a conservative mutation, iv. a missense, v. a nonsense mutation.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Genetic evolution
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS