Here is one set of primers used to amplify a segment of this gene. Select the option that corresponds to the coding sequence, where the reverse primer would anneal. REMEMBER! Reverse primer sequences bind to the coding strand. "HINT* One primer will be found within each bolded region (blue where forward primer would anneal, and yellow where the reverse primer would anneal). Forward primer sequence: 5'-ATGTTGACTC TAACTCGCAT CCGC-3' Reverse primer sequence: 5'- ATGCCCAGA GGGACAAGCT GCCA-3' Reverse primer region on coding strand: O 5-ACCG TCGAACAGGG AGACCCGTA-3 O 3-ACCG TCGTACAGGG AGACCCGTA-5 O 5-TGGC AGCTTGTCCC TCTGGGCAT-3 O 3-TGGA AGCTTGTCCC TCTGGGCAT-5

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Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.