Fig 3.16 EcoRI SacI Kpnl ampR Aval Xmal Smal lacZ BamHI Xbal Sall AccI HincII PstI Sphl HindIII Bam H1 Bam H1 Bam H1 + 1 2 3 4 The ends of the double-stranded DNA fragment above are blunt ends. The directionality of genes contained within the fragment is from left to right (arrow). After digestion by BamH1, which fragment can maintaining the same directionality as the lacz promoter (green segment with arrow on vector)? inserted into the vector cut with BamH1 and Sma 1 O 3 O 4 O1 2

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Fig 3.16
EcoRI
SacI
KpnI
|ampR
Aval
Xmal
Smal
lacZ
BamHI
Xbal
SalI
Accl
HincII
PstI
Sphl
HindIII
Bam H1
Bam H1
Bam H1
1
2
3 4
The ends of the double-stranded DNA fragment above are blunt ends. The directionality
of genes contained within the fragment is from left to right (arrow). After digestion by
BamH1, which fragment can be inserted into the vector cut with BamH1 and Sma 1
maintaining the same directionality as the lacz promoter (green segment with arrow on
vector)?
3
O 4
O1
2
Transcribed Image Text:Fig 3.16 EcoRI SacI KpnI |ampR Aval Xmal Smal lacZ BamHI Xbal SalI Accl HincII PstI Sphl HindIII Bam H1 Bam H1 Bam H1 1 2 3 4 The ends of the double-stranded DNA fragment above are blunt ends. The directionality of genes contained within the fragment is from left to right (arrow). After digestion by BamH1, which fragment can be inserted into the vector cut with BamH1 and Sma 1 maintaining the same directionality as the lacz promoter (green segment with arrow on vector)? 3 O 4 O1 2
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