oth DNA (D) and RNA a. What is the KM b. What is the KM C. What is the Vm d. What is the Vm e. On the graph, c noncompetitiv f. On the graph, uncompetitive
oth DNA (D) and RNA a. What is the KM b. What is the KM C. What is the Vm d. What is the Vm e. On the graph, c noncompetitiv f. On the graph, uncompetitive
Concepts of Biology
1st Edition
ISBN:9781938168116
Author:Samantha Fowler, Rebecca Roush, James Wise
Publisher:Samantha Fowler, Rebecca Roush, James Wise
Chapter9: Molecular Biology
Section: Chapter Questions
Problem 1ACQ: Figure 9.10 You isolate a cell strain in which the joining together of Okazaki fragments is impaired...
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Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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Question
100%
![2200
cleave
2000
1800
I 1/V R
1600
• 1/V D
1400
1200
1000
800
600
400
200
rly.
-1 0 1
5
6.
8.
10
11
y.
1/S (uM)-1
ts resulted in a kcat of 79 min1 and a KM of 87 µM.
urk plots (using kcat for the M-M plot and Vmax for
letely.
1/V (uM x min-1)-1](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fcd7a0374-f414-4e0c-9a39-f312d4f3b754%2F42de4566-a610-4894-8cff-606f8350710c%2F0r49q14_processed.jpeg&w=3840&q=75)
Transcribed Image Text:2200
cleave
2000
1800
I 1/V R
1600
• 1/V D
1400
1200
1000
800
600
400
200
rly.
-1 0 1
5
6.
8.
10
11
y.
1/S (uM)-1
ts resulted in a kcat of 79 min1 and a KM of 87 µM.
urk plots (using kcat for the M-M plot and Vmax for
letely.
1/V (uM x min-1)-1
![1/V (uM x min-1)-1
1. To the right is a Lineweaver-Burk plot for an enzyme that can cleave
both DNA (D) and RNA (R).
2200
2000
1800
a. What is the KM when cleaving DNA?
I 1/V R
1600
• 1/V D
b. What is the KM when cleaving RNA?
1400
1200
C. What is the Vmax when cleaving DNA?
Z 1000
800
d. What is the Vmax when cleaving RNA?
600
e. On the graph, draw an appropriate line for a
400
noncompetitive inhibitor for cleaving DNA. Label clearly.
f. On the graph, draw an appropriate line for an
uncompetitive inhibitor for cleaving RNA. Label clearly.
200
8 10 11
1/S (µM)-1](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fcd7a0374-f414-4e0c-9a39-f312d4f3b754%2F42de4566-a610-4894-8cff-606f8350710c%2Fg8uz45z_processed.jpeg&w=3840&q=75)
Transcribed Image Text:1/V (uM x min-1)-1
1. To the right is a Lineweaver-Burk plot for an enzyme that can cleave
both DNA (D) and RNA (R).
2200
2000
1800
a. What is the KM when cleaving DNA?
I 1/V R
1600
• 1/V D
b. What is the KM when cleaving RNA?
1400
1200
C. What is the Vmax when cleaving DNA?
Z 1000
800
d. What is the Vmax when cleaving RNA?
600
e. On the graph, draw an appropriate line for a
400
noncompetitive inhibitor for cleaving DNA. Label clearly.
f. On the graph, draw an appropriate line for an
uncompetitive inhibitor for cleaving RNA. Label clearly.
200
8 10 11
1/S (µM)-1
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