2200cleave20001800I 1/V R1600• 1/V D140012001000800600400200rly.-1 0 156.8.1011y.1/S (uM)-1ts resulted in a kcat of 79 min1 and a KM of 87 µM.urk plots (using kcat for the M-M plot and Vmax forletely.1/V (uM x min-1)-1 1/V (uM x min-1)-11. To the right is a Lineweaver-Burk plot for an enzyme that can cleaveboth DNA (D) and RNA (R).220020001800a. What is the KM when cleaving DNA?I 1/V R1600• 1/V Db. What is the KM when cleaving RNA?14001200C. What is the Vmax when cleaving DNA?Z 1000800d. What is the Vmax when cleaving RNA?600e. On the graph, draw an appropriate line for a400noncompetitive inhibitor for cleaving DNA. Label clearly.f. On the graph, draw an appropriate line for anuncompetitive inhibitor for cleaving RNA. Label clearly.2008 10 111/S (µM)-1

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Description: 2200cleave20001800I 1/V R1600• 1/V D140012001000800600400200rly.-1 0 156.8.1011y.1/S (uM)-1ts resulted in a kcat of 79 min1 and a KM of 87 µM.urk plots (using kcat for the M-M plot and Vmax forletely.1/V (uM x min-1)-1 1/V (uM x min-1)-11. To the ri

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oth DNA (D) and RNA a. What is the KM b. What is the KM C. What is the Vm d. What is the Vm e. On the graph, c noncompetitiv f. On the graph, uncompetitive

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Concept explainers

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.

Topic Video

Question

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2200
cleave
2000
1800
I 1/V R
1600
• 1/V D
1400
1200
1000
800
600
400
200
rly.
-1 0 1
5
6.
8.
10
11
y.
1/S (uM)-1
ts resulted in a kcat of 79 min1 and a KM of 87 µM.
urk plots (using kcat for the M-M plot and Vmax for
letely.
1/V (uM x min-1)-1

1/V (uM x min-1)-1
1. To the right is a Lineweaver-Burk plot for an enzyme that can cleave
both DNA (D) and RNA (R).
2200
2000
1800
a. What is the KM when cleaving DNA?
I 1/V R
1600
• 1/V D
b. What is the KM when cleaving RNA?
1400
1200
C. What is the Vmax when cleaving DNA?
Z 1000
800
d. What is the Vmax when cleaving RNA?
600
e. On the graph, draw an appropriate line for a
400
noncompetitive inhibitor for cleaving DNA. Label clearly.
f. On the graph, draw an appropriate line for an
uncompetitive inhibitor for cleaving RNA. Label clearly.
200
8 10 11
1/S (µM)-1

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