13. Southern blotting is separation of DNA by agarose electrophoresis and probing with a labeled primer. Byanalogy, Eastern blotting is:A a method that analyzes nucleotides and small RNA fragmentsB. a method that analyzes ribosomal RNAC. a method that analyzes transfer RNAD. a method that analyzes messenger RNAE. all of the aboveF none of the above

The 'blot' in the different blotting techniques (southern, northern, western and eastern blotting)…

Answered: 13. Southern blotting is separation of…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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4
8.
It is difficult to use a restriction enzyme that cuts (shown as *) within one of these restriction sites for cloning purposes O a. GCA*TGC O b. GCGCGCG*C Oc.C*GCG O d. *AAAATTTT O e. AGC*T
1. Write if the statement is true and modify the statement with the correct answer if its false a. E. coli strains used in blue-white screening produce blue color complexes because their beta-galactosidases are activated via alpha-complementation. b. If you are a researcher trying to identify a gene that is responsible for a trait of interest, the first thing that you would do is to make a review of the literature that is available. c. The polylinker region in plasmids is where specific DNA sequences are cut by non-specific restriction enzymes. d. Blue-white screening is one of the last steps involved in determining if the gene of interest has been inserted successfully in plasmids.
Which of the following enzymes is mainly responsible for recognizing the PAM sequence in the bacterial genome O a. Cas 6 b. Cas 2 O C. Cas 9 Cas 1 O e. Cas 3 Which one of the following is considered as a kind of genetic engineering modification O a. Inserting a foreign gene from one species into another Knocking out i genes Oc. The production of genetically modified organism O d. Altering an existing gene by mutation to change its product. O e. All the above applies. In the example we studied, which one of the following methods was used to fix the sickle cell anemia O a. Anti-sense RNA O b. Directed mutagenesis O C. Anti-sense oligonucleotides O d. Random mutagenesis e.
To quantify the transcription product of a specific gene in E. coli, you may use O a. Topoisomerase I O b. Northern blotting С. Primer extension O d. Foot-printing
4. Once you had your DNA sample extracted from your cells, why did we add the yellow Master Mix solution? Specifically explain the roles of the following: a. primers b. Taq polymerase C. Mg (Why would we add this back in when earlier we wanted to remove Mgʻ*?). d. individual nucleotides eelsta yoa
1. A short homologous sequence called the macrohomologies which connects break ends of the strands. a. True b. False Answer: 2. This type of DNA repair can recognize multiple damaged part in the DNA sequence and repair it. a. Nucleotide repair b. Strand break repair c. Base excision repair d. Base nucleotide replacement Answer:
56. In gel electrophoresis, DNA is made visible with 5o. Use the following information to answer the next question. autoradiographic blue. a. Na - K pump situated in the neural membrane b. RFLP dye. C. agarose. d. polyacrylamide gel. Outside Inside ----K* diffusion e. ethidium bromide. Na carier 57. Gene therapy alters a patient's genome by Na* diffusion - adding bacteria to the genome. excising nucleotides. adding a corrected gene. engineering transgenic tissue. a. b. c. Which of the following correctly describes the ion flow into or out of a neural fibre during repolarization? d. e. bioremediation. K* flow out K* flow in a. b. 58. Use the following information to answer the next question. Na' flow out Na' flow in c. d. Neuron none of these e. Use the diagram given below to answer the next question. 61. Human Brain Myelinated neurons conduct impulses faster than unmyelinated neurons because in myelinated fibers, sodium gates open only a dendrite labeled 1 dendrite labeled 4 a. 2- b.…
2. A. B. C. The DNA of each species has a different base composition. Find the base composition of each species, using what you know about complementary base pairing. Human DNA is approximately 20% C. G = % % A = % In the bacteriophage DX174, the DNA is approximately 26% A. C = % G = % A = T= Sea urchin DNA is approximately 18% G. C = % T = % T= % % 64
7. Electroporation is a physical method of transferring genetic material into the cells. True False 8. You have completed an in vitro mutagenesis experiment to create an G to T mutation in the coding sequence of the heat shock protein gene. This gene is cloned in a plasmid and you want to confirm that the mutation was correctly introduced. Which sequencing technology be most appropriate to use practically and economically: Microarray Sanger sequencing RNA sequencing Exon sequencing

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