e CRISPR associated protein tinal

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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9. The CRISPR associated protein Cas9 is now being used as a molecular tool for genome
editing
C. In a cell, what happens to a gene that has been cut by a genome editing system? It is
repaired using either non-homologous end joining (NHEJ) or homology dependent
repair (HDR)
D. The Cas9 protein requires an RNA molecule to carry out genome editing. What
sequence requirements does this RNA have?
E. Name two limitations of CRISPR/Cas9 genome editing.
Transcribed Image Text:9. The CRISPR associated protein Cas9 is now being used as a molecular tool for genome editing C. In a cell, what happens to a gene that has been cut by a genome editing system? It is repaired using either non-homologous end joining (NHEJ) or homology dependent repair (HDR) D. The Cas9 protein requires an RNA molecule to carry out genome editing. What sequence requirements does this RNA have? E. Name two limitations of CRISPR/Cas9 genome editing.
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