EcoRI Sacl Kpnl Aval ampR Xmal Smal lacZ BamHI Xbal Sall Accl HinclI Pstl Sphl HindIII Bam H1 Bam H1 Bam H1 1 2 3 4 The ends of the double-stranded DNA fragment above are blunt ends. The directionality of genes contained within the fragment is from left to right (arrow). After digestion by BamH1, which fragment can be inserted into the vector cut with BamH1 and Sma 1 maintaining the same directionality as the lacz promoter (green segment with arrow on vector)? O 2 01 O 4

Human Anatomy & Physiology (11th Edition)
11th Edition
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Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Fig 3.16
EcoRI
SacI
ampR
KpnI
Aval
Xmal
Smal
lacZ
BamHI
Хbal
Sall
Аccl
HincII
PstI
SphI
HindIII
Bam H1
Bam H1
Bam H1
+
1
3 4
The ends of the double-stranded DNA fragment above are blunt ends. The directionality
of genes contained within the fragment is from left to right (arrow). After digestion by
BamH1, which fragment can be inserted into the vector cut with BamH1 and Sma 1
maintaining the same directionality as the lacZ promoter (green segment with arrow on
vector)?
2
3.
O1
4
Transcribed Image Text:Fig 3.16 EcoRI SacI ampR KpnI Aval Xmal Smal lacZ BamHI Хbal Sall Аccl HincII PstI SphI HindIII Bam H1 Bam H1 Bam H1 + 1 3 4 The ends of the double-stranded DNA fragment above are blunt ends. The directionality of genes contained within the fragment is from left to right (arrow). After digestion by BamH1, which fragment can be inserted into the vector cut with BamH1 and Sma 1 maintaining the same directionality as the lacZ promoter (green segment with arrow on vector)? 2 3. O1 4
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