In Western Blotting a complex ________ mixture is separated by ________ then selectively identified by _______ binding and catalytic staining.Select one:a.Virus, Size, Macrophageb.Virus, Size, Antibodyc.Protein, pI, Ligandd.Protein, Size, Antibodye.None of these.

Answered: In Western Blotting a complex ________…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

In Western Blotting a complex ________ mixture is separated by ________ then selectively identified by _______ binding and catalytic staining.

Select one:

Virus, Size, Macrophage

Virus, Size, Antibody

Protein, pI, Ligand

Protein, Size, Antibody

None of these.

Expert Solution

Step 1

The blot analysis techniques are powerful methods used for the detection of specific biomolecules in samples containing mixtures of biomolecules. They are auxiliary to gel electrophoresis.

Blotting techniques have exceptional resolving power. It is applied to those biomolecules that can stably adhere to support material and still have the ability to bind their cognate ligand. The biomolecules are separated on the basis of their size or charge and then are transferred to the membrane.