Which of the following statements about base editing is correct? 1. It involves nCas9. 2. The end result of the complete process is the conversion of GC to CU. 3. The "chemistry on the genome" is performed by deaminase. 4. UGI is fused to Cas9 in order to prevent any DNA repair from occurring. O A. 1, 2 and 3 B. 1 and 3 O C. 2 and 4 D. 4 only E. All of 1, 2, 3 and 4 are correct

Which of the following statements about base editing is correct? 1. It involves nCas9. 2. The end result of the complete process is the conversion of GC to CU. 3. The "chemistry on the genome" is performed by deaminase. 4. UGI is fused to Cas9 in order to prevent any DNA repair from occurring. O A. 1, 2 and 3 B. 1 and 3 O C. 2 and 4 D. 4 only E. All of 1, 2, 3 and 4 are correct

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications

Section: Chapter Questions

Problem 3TYK: Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning...

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1. Choose the phrase from the right column that best fitsthe term in the left column.a. transformation 1. requires supplements in medium forgrowthb. conjugation 2. a method for mutagenizing genes inbacterial genomesc. transduction 3. small circular DNA molecule that canintegrate into the chromosomed. lytic cycle 4. the core genes that define a bacterialspecies plus all of the genes unique toindividual strainse. lysogeny 5. transfer of DNA requiring directphysical contactf. episome 6. integration of phage DNA into thechromosomeg. auxotroph 7. infection by phages in which lysis ofcells releases new virus particlesh. pangenome 8. transfer of naked DNAi. gene targeting 9. transfer of DNA between bacteria viavirus particles
DNA molecular with complementary sticky ends associate by (a) covalent bonds (b) hydrogen bonds (c) ionic bonds (d) disulfide bonds (e) phosphodiester linkages
Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors? a. The plasmid must have resistance to accept DNA inserts. b. They allow the detection of plasmids that contain an inserted DNA fragment. c. They ensure the presence of the ori site. d. They ensure that the plasmid can be cut by a restriction enzyme. e. They allow identification of bacteria that have taken up a plasmid.
1. Which of these methods will allow cell counting and segregation? a. Flow Cytometry b. Western Blot c. Immunocytochemistry d. ELISA 2. Which of the following is not a consequence of a CRISPR/Cas9-mediated double strand break that occurred in the absence of a homologous chromosome? a.The target sequence will be repaired without errors b.The target sequence will be repaired with errors c.The target sequence will incur deletions d.The target sequence will incur insertions.
Match the concept in column A to the concept in column B. Write the letter of the correct answer on the blank provided. A В a. Change that affects the ability of DNA to be transcribed to 1 Karry Mullis RNA without directly modifying the DNA sequence. b. transformation epigenetic modification 18-25 nucleotides C. can be transformed d. genetic engineering e. primers f. biotechnology g. regulated repressor h. Nucleotide 3 4 recombination Frederick Griffith forward primer competent cells Karoly Ereke 6 7 8 sequences are exchanged between two identical DNA molecules i. Repressible negative regulation j. Complementary to the 3' end of the recombinant DNA 10 tryp operon anti-sense strand k. Complementary to the 3' end of sense strands I. Polymerase chain reaction
Retrotransposons are mediated by the enzyme O a. transposase O b. RISC O c. retrotransposase O d. retrotranscriptase O e. reverse transcriptase
1 and/or 2 please
Transformation can be transient or stable, depending on how long the foreign DNA persists in the cell. Lütfen birini seçin: a. True O b. False
1. This complex assembles and organizes nucleosomes and contributes to gene repression Group of answer choices a. SWR1 Complex b. ISWI Complex c. SWI/SNF Complex d. SWI Complex 2. A DNA lesion occurring when the deoxyribose molecule loses an adenine or a guanine base. Group of answer choices a. Depurination b. Deamination c. Depyrimination d. Denaturation 3. The difference between a nucleoside and nucleotide is: Group of answer choices a. Nucleotides contains deoxyribose sugar and nucleosides contain ribose sugar b. A nucleoside consists of the sugar with a nitrogenous base, whereas a nucleotide has sugar with a nitrogenous base and phosphate groups attached to the sugar. c. A nucleotide consists of the sugar with a nitrogenous base, whereas a nucleoside has sugar with a nitrogenous base and phosphate groups attached to the sugar. d. Nucleotides are involved in eukaryotic DNA replication, while nucleosides are used in bacterial DNA replication
1.What type of mutation is seen here? WT: 5′-AUG GCU AGA GUU GAA AAA-3′ Mutant: 5′-AUG GGU AGA GUU GAA AAA-3′ Group of answer choices a. Transition b. Tranversion c. Insertion d. Deletion 2. Dr. Doom wants to prevent a cell from entering the lysogenic pathway, which gene controlled by PRM would Dr. Doom target for inactivation? Group of answer choices a. cII b. cro c. cI d. Q
Match items in Column A with most related ones in B. Write the letters on the space provided.
Section B: True / False (5 marks) 1. Primase has a DNA dependent RNA polymerase activity. 2. 7-methylguanosine cap is added to the 5' end of the DNA after replication in eukaryotes. 3. snRNA is produced in transcription. 4. Initiator tRNA enter ribosome with the help of EF-Tu. 5. At low oxygen partial pressure Po2, myoglobin has lower oxygen affinity than hemoglobin.