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- 1. Photoreactivation destroys the covalent bond by using the light energy from the UV light source that does not occur in humans. a. True b. False Answer: 2. Single-stranded annealing can cause loss of genetic information. a. True b. False Answer: 3.) This family of enzymes removes the damaged base before a nucleotide gap can be filled by DNA polymerase. a. Ligase b. Polymerase c. Tropo-isomerases d. Glycosylases Answer:What does it mean to say thhat extension by DNA polymerase III proceed 5'---3'? A. The 5' end of a DNA polymerase molecule attaches to the 3' end of primase. B. DNA polymerase adds nucleotides to a growing strand, moving in the 5' to 3' direction. C. DNA polymerase seals nicks as it moves along a DNA strand toward the 3' end. D. DNA polymerase can only synthesize DNA at the 5' end of an existing strand of DNA.1. DNA polymerase A. Short DNA sequences synthesized on the lagging strand 2. replication fork B. Replicated discontinuously in the 5' to 3' direction 3. Chromosome C. The structure made of DNA that carries genes 4. free nucleotides D. The Cs, Gs, Ts, & As ready to be added to growing chain 5. Topoisomerase F. The Y-shaped point of origin for DNA replication 6. Okazaki Fragment G. Manages the overwinding of DNA ahead of the fork 7. Leading strand H. Lays down nucleotides in DNA replication 8. Lagging strand I. replicated continuously in the 3' - 5' direction
- I. Unwinding of DNA and formation of replication fork II. Proofreading of strands and replacing any errors during the synthesis. III. Synthesizing a new strand that matches the template, by extending the primer via the addition of he nucleotides to the 3' end. IV. Formation of semiconservative strands Which stage use/s DNA polymerase? A. I and II B. II and III C. Il only D. Ill only Which of the following sequence shows the correct process of DNA replication? A. I, I, III, IV B. I, II, IV, III C. I, III, IV, II D. I, III. II, IVThere are several factors that contribute to high fidelity of DNA replication. Which of the following statements is INCORRECT? DNA polymerase I has a 3'-5' exonuclease editing activity. O b. RNA primers are used instead of DNA primers. There are balanced levels of dNTPs inside the cell. O C. O d. DNA polymerase III has a 3'-5' exonuclease editing activity. O e. Base-pair geometry plays important role for maintaining the high fidelity of DNA synthesis.34. What direction does DNA polymerase extend and proofread DNA? A. Extend: 5'--3' and 3'--5'; Proofread: 3'--;5' B. Extend: 5'--3'; Proofread: 3'--5' C. Extend: 5'--3'; Proofread: 5'--3' D. Extend: 5'--3' and 3'--5'; Proofread: 5'--3' and 3'--5' E. Extend: 5'--3'; Proofread: 5'--3' and 3'--5'
- 40.Would it be possible to start synthesizing the daughter DNA strand without assembling the RNA primer first? Why? Why not? A.Yes, because the 5' PO4 is already present in the DNA strand which will be used as a template. B.No, because the RNA primer which contains the free 3' OH in its ribose has to be synthesized by primase first. C.No, because the RNA primer which contains the free 5' PO4 in its ribose will not be synthesized by primase. D.Yes, because the 3' OH is already present in the DNA strand which will be used as a template.The function of gamma (y) complex of DNA polymerase III is to Select one: O a. to unwind double strand DNA (template DNA). O b. to associate the core DNA polymerase llI with template DNA. O c. to stimulate 5' to 3' exonuclease activity O d. to upload beta subunit of DNA polymerase III to template DNA.23. What process best describes the action of Taq DNA polymerase? a. Catalyzes hydrolysis of phosphodiester bond b. Catalyzes an oxidation-reduction reaction c. Catalyzes the formation of a phosphodiester bond
- 5) Both DNA polymerase (any DNA polymerase) and ligase catalyze the formation of a bond between nucleotides, but these two enzymes do NOT catalyze the same reaction. Briefly describe the differences between the reaction catalyzed by the polymerase activity of a DNA polymerase and the reaction catalyzed by ligase.1. For each of the items below, give a brief description (indicate function for enzymes) and indicate in which process (replication, transcription, or translation) it is involved with. Description/Funetion catalyzes the unwinding of DNA strands Process involved in helicase replication 1. RNA primer 2. leading strand 3. RNASE 4. anti-sense strand 5. anticodon 6. RNA polymerase II 7. DNA polymerase 8. promoter site 9. DNA ligase 10. exit site3b) Briefly explain what telomerase does, how it accomplishes what it does, and why that allows a cell to completely and accurately replicate the ends of linear DNA molecules. (please note that the question does not ask you to explain the entire process of replication of the end of a linear DNA strand, it only asks about the function of telomerase in this process)