5. The downstream process for a recombinant protein begins with 100 liters of clarified lysate. At the end of the purification steps, two liters protein solution are collected. The total mass of protein is measured in mg/ml for both solutions, and the specific activity of the recombinant protein in both solutions are measured in U/ml. The compositions of the two streams are given in Table 1. A solution of pure recombinant protein has a specific activity of 44.0 U/mg. Calculate the purity, specific activity, % yield, and fold purification for the clarified lysate and final protein solution. Table 1. Protein data for clarified lysate and final product solution Specific Activity U/ml Volume L Total Protein Solution mg/ml Clarified Lysate 100 0.36 2.2 Protein Solution 2 1.11 43.2

Biochemistry
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Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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5. The downstream process for a recombinant protein
begins with 100 liters of clarified lysate. At the end of the
purification steps, two liters protein solution are collected.
The total mass of protein is measured in mg/ml for both
solutions, and the specific activity of the recombinant
protein in both solutions are measured in U/ml. The
compositions of the two streams are given in Table 1. A
solution of pure recombinant protein has a specific activity
of 44.0 U/mg.
Calculate the purity, specific activity, % yield, and fold
purification for the clarified lysate and final protein
solution.
Table 1. Protein data for clarified lysate and final product
solution
Specific Activity
U/ml
Volume L
Total Protein
Solution
mg/ml
Clarified Lysate
100
0.36
2.2
Protein Solution
1.11
43.2
Transcribed Image Text:5. The downstream process for a recombinant protein begins with 100 liters of clarified lysate. At the end of the purification steps, two liters protein solution are collected. The total mass of protein is measured in mg/ml for both solutions, and the specific activity of the recombinant protein in both solutions are measured in U/ml. The compositions of the two streams are given in Table 1. A solution of pure recombinant protein has a specific activity of 44.0 U/mg. Calculate the purity, specific activity, % yield, and fold purification for the clarified lysate and final protein solution. Table 1. Protein data for clarified lysate and final product solution Specific Activity U/ml Volume L Total Protein Solution mg/ml Clarified Lysate 100 0.36 2.2 Protein Solution 1.11 43.2
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