Suppose you have a sample of plasmid DNA that you want to analyze by performing a restriction digest and a gel. You have 200 μl of your DNA sample, and the DNA concentration is 40 µg/ml. The uncut DNA is 10 kb long, and you expect it to be cut into fragments of 6 kb, 3 kb, and 1 kb. You want to cut enough DNA to give you 50 ng in the 1-kb band, and then load it all on the gel. Show your calculations for everything you would put into the restriction digest tube. The stock concentration of the restriction enzyme is 5 Units/μl, and one unit of enzyme cuts one µg of DNA.
Suppose you have a sample of plasmid DNA that you want to analyze by performing a restriction digest and a gel. You have 200 μl of your DNA sample, and the DNA concentration is 40 µg/ml. The uncut DNA is 10 kb long, and you expect it to be cut into fragments of 6 kb, 3 kb, and 1 kb. You want to cut enough DNA to give you 50 ng in the 1-kb band, and then load it all on the gel. Show your calculations for everything you would put into the restriction digest tube. The stock concentration of the restriction enzyme is 5 Units/μl, and one unit of enzyme cuts one µg of DNA.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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solve this with step by step calcuations please
Transcribed Image Text:Suppose you have a sample of plasmid DNA that you want to analyze by performing a restriction digest and a
gel. You have 200 μl of your DNA sample, and the DNA concentration is 40 µg/ml. The uncut DNA is 10 kb
long, and you expect it to be cut into fragments of 6 kb, 3 kb, and 1 kb. You want to cut enough DNA to give
you 50 ng in the 1-kb band, and then load it all on the gel. Show your calculations for everything you would
put into the restriction digest tube. The stock concentration of the restriction enzyme is 5 Units/μl, and one
unit of enzyme cuts one µg of DNA.
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