Using Beer's Law, please calculate the concentration of your sample if your absorbance reading at 260 were 0.8 absorbance units. (assume a path length of 1, and use the extinction coefficient for double-stranded DNA
1. Using Beer's Law, please calculate the concentration of your sample if your absorbance reading at 260 were 0.8 absorbance units. (assume a path length of 1, and use the extinction coefficient for double-stranded DNA).
2. In the miniprep procedure, how does one separate DNA from proteins & sugars in the cell lysate?
3. During the DNA miniprep, you must first resuspend your bacterial pellet (typically by vortexing). However, once you add the lysis buffer, it is important that you do not vortex anymore. Why is it important not to vortex your sample after addition of lysis buffer?
4. If the plasmid has been taken up by the bacterial cells, but the TIR protein can not be expressed, even after induction with IPTG, which of the following parts of the plasmid might be faulty?
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