Oops!  Your pipettor wasn’t set correctly and you only pipetted 87 µl of a 10-7 dilution when plating serial dilutions.  But, you noted this error in your lab notebook so you could correctly perform the calculation.  When this plate was grown, you counted 147 colonies on the plate.  What was the concentration of bacteria in the original, undiluted suspension?

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Q: Why is polyacrylamide gel is used in electrophoresis?

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Q: a food sample with an unknown number of contaminating bacteria was serial diluted to 10-6. the 10-6…

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Q: Dilution Problem. A culture was diluted as follows: (1) 50mL are added to 100mL of water. (2) 1mL…

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Q: How should you prepare your running buffer?

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Q: Each liter of an intranasal antibiotic solution contains 160 mg of gentamicin. You have 2-mL…

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Q: If the 10-9 plate was counted to have 155 colonies on it, would the CFU/mL be 1.55*1011? If not,…

A: CFU  Colony forming unit it a method to calculate the number of microorganisms in a culture media.

Q: ) If you counted 500 CFU’s in the petri dish for number 6 and 5000 CFU’s in petri dish for number 7,…

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Q: A student performed an invertase activity assay on samples from a purification.  All reactions…

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Q: You perform the Methyl-Red(MR)& Voges-Proskauer(VP)test and gather the followingresults:…

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Q: One hundred microliters (0.100 ml) of a 10-5 dilution of a bacterial suspension plated on an agar…

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Q: Prepare the following LB media with antibiotic added. A 100ml of LB media with 25 micro/ml of Amp…

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Q: Assume that you counted 67 plaques on a bacterial plate where 0.1ml of a 10-5 dilution of phage was…

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Q: You have a 50X stock solution of TAE buffer, but you need 2 L of a 1X solution for electrophoresis.…

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Q: If you count 70 colonies from a urine culture obtained using 0.001ml calibrated loop, how many…

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Q: why In spread-plate technique, the bacterial suspension volume  should not exceed 0.1ml.

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Q: The stool samples submitted by the two friends were enumerated following enrichment. The technician…

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Q: Determine the concentration of L. monocytogenes enumerated from the apple slice based on the…

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Q: CFU/mL

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Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.