The stool samples submitted by the two friends were enumerated following enrichment. The technician prepared serial dilutions. A volume of 1 mL of stool was mixed with 1 mL of diluent. From this dilution, they then took 1 mL and added it into 1 mL of diluent. The operation was repeated 5 more times. After finishing the serial dilution, 100 μ�L has been spread on different TSA plates. Following 24h incubation, the technician counted 23 colonies at the dilution 4.   What is the bacterial concentration in the enriched culture? [1 mark]

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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The stool samples submitted by the two friends were enumerated following enrichment. The technician prepared serial dilutions. A volume of 1 mL of stool was mixed with 1 mL of diluent. From this dilution, they then took 1 mL and added it into 1 mL of diluent. The operation was repeated 5 more times. After finishing the serial dilution, 100 μ�L has been spread on different TSA plates. Following 24h incubation, the technician counted 23 colonies at the dilution 4.

 

What is the bacterial concentration in the enriched culture? [1 mark] 

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