Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
The PD# of your culture is 165. You plated 3745 cells per well. You have recovered 12,655 cells. What's the new PD#?
Expert Solution
Step 1
The passage number of a cell culture is a record of the number of times the culture has been subcultured, i.e. harvested and reseeded into multiple ‘daughter’ cell culture flasks.
The population doubling (PD or pd) number is the approximate number of doublings that the cell population has undergone since isolation. This is a more meaningful estimate of the age of a finite cell line. When the split ratio used to passage cells is 1:2 the passage number is equal to the PD
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