The fallowing series of dilution was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4. Calculate the dilution factors for each tube. What is the cell concentration in the original specimen? Calculate the total number of cells in tube number 2.
The fallowing series of dilution was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4. Calculate the dilution factors for each tube. What is the cell concentration in the original specimen? Calculate the total number of cells in tube number 2.
Chapter12: Parenteral Equipment And Supplies
Section: Chapter Questions
Problem 24RQ
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Transcribed Image Text:**BONUS (15 points)**
The following series of dilutions was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from tube number 4.
1. Calculate the dilution factors for each tube.
2. What is the cell concentration in the original specimen?
3. Calculate the total number of cells in tube number 2.
(Note: The image contains text that guides students in calculating bacterial concentrations through a serial dilution process, presenting a practical application in microbiology.)
Transcribed Image Text:The image illustrates a serial dilution process using five test tubes:
1. **Test Tube 1** contains the initial sample with a volume of 9.5 ml. A 0.5 ml aliquot from the sample is transferred to the next tube.
2. **Test Tube 2** contains 9 ml after adding the 0.5 ml from the original sample. Then, 1 ml is taken and transferred to the next tube.
3. **Test Tube 3** also starts with 9 ml after a 1 ml transfer from the previous tube. Another 1 ml is transferred to the next tube.
4. **Test Tube 4** follows the same process, starting with 9 ml, and 1 ml is transferred to the next tube.
5. **Test Tube 5** ends with 9 ml after receiving 1 ml from Test Tube 4.
After the final dilution, 0.3 ml is plated, resulting in the formation of 62 colonies. This diagram exemplifies how serial dilutions can be used to estimate the concentration of viable microorganisms in a sample.
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