**BONUS (15 points)**The following series of dilutions was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from tube number 4.1. Calculate the dilution factors for each tube.2. What is the cell concentration in the original specimen?3. Calculate the total number of cells in tube number 2.(Note: The image contains text that guides students in calculating bacterial concentrations through a serial dilution process, presenting a practical application in microbiology.) The image illustrates a serial dilution process using five test tubes:1. **Test Tube 1** contains the initial sample with a volume of 9.5 ml. A 0.5 ml aliquot from the sample is transferred to the next tube.2. **Test Tube 2** contains 9 ml after adding the 0.5 ml from the original sample. Then, 1 ml is taken and transferred to the next tube.3. **Test Tube 3** also starts with 9 ml after a 1 ml transfer from the previous tube. Another 1 ml is transferred to the next tube.4. **Test Tube 4** follows the same process, starting with 9 ml, and 1 ml is transferred to the next tube.5. **Test Tube 5** ends with 9 ml after receiving 1 ml from Test Tube 4.After the final dilution, 0.3 ml is plated, resulting in the formation of 62 colonies. This diagram exemplifies how serial dilutions can be used to estimate the concentration of viable microorganisms in a sample.

The viable count method is a commonly used technique in a microbiology laboratory to access the…

The fallowing series of dilution was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4. Calculate the dilution factors for each tube. What is the cell concentration in the original specimen? Calculate the total number of cells in tube number 2.

The fallowing series of dilution was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4. Calculate the dilution factors for each tube. What is the cell concentration in the original specimen? Calculate the total number of cells in tube number 2.

Principles Of Pharmacology Med Assist

6th Edition

ISBN:9781337512442

Author:RICE

Publisher:RICE

Chapter12: Parenteral Equipment And Supplies

Section: Chapter Questions

Problem 24RQ

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The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution Plate 1 10 dilution Plate 2 10 dilution Plate 3 107 dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* 382 83 10 2 0
The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate 1 10 Plate 2 10 Plate 3 10 dilution dilution dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 10 dilution -6 *Too many to count Number of colony forming units (CFU) TMTC* TMTC* 840 28 19 1
The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 1 mL aliquot from each dilution was mixed with warm molten agar and poured into a Petri dish. The numbers of bacterial colony forming units (CFU) after overnight incubation are shown in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution -3 Plate 1 10 dilution Plate 2 10-4 dilution Plate 3 10-5 dilution Plate 4 10 dilution Plate 5 10 dilution -8 Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* 1,957 65 21 1 0 m
The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 1 mL aliquot from each dilution was mixed with warm molten agar and poured into a Petri dish. The numbers of bacterial colony forming units (CFU) after overnight incubation are shown in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution Plate 1 10 dilution Plate 2 10 dilution -5 Plate 3 10 dilution Plate 4 10 dilution Plate 5 107 dilution -8 Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* TMTC* TMTC* 867 154 18
A laboratory technician performed a series of 10 fold serial dilutions shown below. he plated 0.1 mL from each dilution tube onto NA and got the follow numbers per plate dilution 1:10 1:10 1:100 1:1000 1:10000 1:100000 colony count >1000 >1000 808 303 38 3 calculate the concentration of bacteria in the undiluted stock (in CFU/mL)
What is the dilution factor in the task given if the total viable cells is 140 [28×5(squares)] and the total nonviable cells is 50 [10x5(squares)]?
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Calculate the concentration of bacteriophage in the original culture from the following data. Be sure to include units. Show dilution factors for each test tube. Show the final dilution factor for test tube number 4. Show all your math. 0.1 ml 0.01 ml 0.001 ml 0.1 ml 1.0 ml plated 9.9 ml 9.99 ml 9.999 mi 9.9 m plaques Original Culture Test Test Test Test Tube #1 Tube #2 Tube #3 Tube #4 Dilution Factor: Concentration of Phage:
Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,000
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