The number of bacterial cells in a culture broth is to be determined by a culturetechnique.Serial dilutions were performed and a 1 mL aliquot from each dilution was mixed withwarm molten agar and poured into a Petri dish. The numbers of bacterial colonyforming units (CFU) after overnight incubation are shown in the table below.What is the number of colony forming units per mL of the culture broth? Choose onlythe most appropriate plate for your calculation. Give your answer as the number only(do not add text for the units). You may use scientific notation with the format1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer awhole number.)Plate DilutionPlate 1 10 dilutionPlate 2 10 dilution-5Plate 3 10 dilutionPlate 4 10 dilutionPlate 5 107 dilution-8Plate 6 100 dilution*Too many to countNumber of colony forming units (CFU)TMTC*TMTC*TMTC*86715418

Here, we will explore the process of determining the number of bacterial colony-forming units (CFUs)…

Answered: The number of bacterial cells in a…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Similar questions

The following is result from a standard plate count. What is the cell density of the undiluted culture? Please follow all SPC rules. (TNTC: too numerous to count)
The total number of cells in a culture is counted using the trypan blue exclusion assay and is found to be 6.8 x 106 cells/ml. Each well in a 6 well plate requires 2 x 105 cells. How should the solution be diluted so that 1ml can be added to each well?
A 0.00001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 223 colonies of bacteria grow on the petri dish. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer to two decimal places using exponential notation. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed. Any time more than 1 mL is transfered, a concentration is being performed.
Streptococcus pyogenes is a Gram-positive human pathogen. It has a cell diameter of 600nm. How many entire Streptococcus pyogenes could fit across this computer screen? (The screen measures 0.24 meters across.) Write just the number with any needed commas. Do not include units.
Describe how you would make 10 ml of a 1:500 dilution of bacteria by performing TWO SEPARATE DILUTIONS in series? Consider the accuracy of the pipetting so that you don’t make a single dilution that is too large.
A culture of S. cerevisea has an overnight OD of 4.5 (1.0 OD is approx 1.0x107 cells/ml) You will be plating 100µl onto agar and want the final count of colonies on the plate to be around 300 colonies. How much of the 4.5 OD culture must you use to get a 500µl subdilution (with sterile water), so that you have diluted enough to get approx 300 colonies per 100ul
When testing the efficacy of an antibiotic against bacteria on an agar plate, it is important to spread the bacteria evenly across the plate before you add the antibiotic. Why do the bacteria need to be applied evenly? None of the following are true All of the following are true If you add more bacteria in some areas compared to others, it might obscure the effect of the antibiotic If the area right next to the antibiotic initially received relatively few bacterial cells, that might lead you to overestimate the effectiveness of the antibiotic If the area right next to the antibiotic initially received a relatively large number of bacterial cells, that might lead you to underestimate the effectiveness of the antibiotic
A bacterial suspension with a population of 6.3 x 107cells/mL was diluted as follows: 4 mL was removed and added to 16 mL of diluent. This was again diluted 1 in 300. Determine the concentration of cells after EACH dilution. Concentration after the 1st dilution = 6.3 x 107 cells/mL x 1/5 = 12.6 x 106 cells/mL Concentration after the 2nd dilution = 12.6 x 106 cells/mL x 1/300 = 42000 cells/mL
Why should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?
You must write up your OWN dichotomous key for all the possible unknown organisms listed below . The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures . Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes Imp Note - the test should be performed using any of the following tests stated below. Bile Escalin test, Oxidaze test, Blood Agar , Catalese Test, Mannitol salt agar . Thank you !
If you plated 0.1mL of a 103 dilution and you obtained 235 colonies on your agar plate, how many CFUs per mL were in your original sample? If a bacterium can divide once an hour, then after 5 hours, 1 bacterium will have yielded ______ bacteria
Calculate the concentration of bacteriophage in the original culture from the following data. Be sure to include units. Show dilution factors for each test tube. Show the final dilution factor for test tube number 4. Show all your math. 0.1 ml 0.01 ml 0.001 ml 0.1 ml 1.0 ml plated 9.9 ml 9.99 ml 9.999 mi 9.9 m plaques Original Culture Test Test Test Test Tube #1 Tube #2 Tube #3 Tube #4 Dilution Factor: Concentration of Phage:

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